Masters Degrees (Microbial, Biochemical and Food Biotechnology)
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Classification and spoilage characteristics of Chryseobacterium isolates from fish
(University of the Free State, 2019)The genus Chryseobacterium was initially reported to have only six species and the number has increased rapidly over the years. The genus is represented by a total of 113 species at present. Species belonging to the genus ... -
The development of a CRISPR-Cas9 gene editing system for Cryptococcus deneoformans
(University of the Free State, 2019-01)The pathogenic yeasts, Cryptococcus neoformans and C. deneoformans, are responsible for potentially fatal meningoencephalitis in immunocompromised individuals, most notably in patients who have AIDS. Only three drugs are ... -
Studying the regulation of immune signalling molecules related to immunity during Avibacterium paragallinarum infection
(University of the Free State, 2019-06)Infectious coryza is a contagious and acute upper respiratory poultry disease caused by Avibacterium paragallinarum, that plagues predominantly layers but also affects broiler breeds. The chicken’s immune response to Av. ... -
The development of a wide range CRISPR-Cas9 gene editing system
(University of the Free State, 2019-01)CRISPR is a revolutionary method to effectively and efficiently alter the genomic make-up of an organism. Unlike any other genetic engineering tool or technique, CRISPR is remarkably cheaper, simpler and faster to perform. ... -
Antibody fragments as a possible therapeutic treatment for infectious bronchitis in poultry
(University of the Free State, 2018-09)Infectious bronchitis virus (IBV), a coronavirus, is the etiological agent for infectious bronchitis (IB), an acute respiratory disease of poultry. Infectious bronchitis is a notifiable disease, and taking in consideration ... -
Changes in microbial ecology during poultry production
(University of the Free State, 2000-03)Microorganisms, especially pathogens, play an important role in the deterioration of poultry meat and its products causing spoilage or food poisoning. The meat renders an ideal medium for the growth and progression of ... -
The utilisation of used and other fats by fungi
(University of the Free State, 2000-09)English: In 1997, Jeffery and co-workers discovered that when Mucor circinelloides t. circinelloides CBS 108.16 was cultivated on 30g/1 sunflower oil and 10g/1 sodium acetate, an improved utilisation of the oil, doubling ... -
Mucoralean fungi present in soil from arid regions in South Africa
(University of the Free State, 1999-11)English: The aim of the first part of the study was to investigate the ecological niche of mucoralean fungi in arid soil, with specific reference to the position these fungi occupy in the biogeochemical cycle of nitrogen. ... -
Ophiostoma species from hardwood sources in South Africa
(University of the Free State, 2001-10)English: The ophiostomatoid fungi are an economically important group of fungi, known for their ability to stain sapwood and cause tree diseases. In recent years, certain species in the group have also been considered as ... -
The growth kinetic characterisation of Saccharomyces cerevisiae strains transformed with amylase genes
(University of the Free State, 2002-03)English: The direct fermentation of starch to ethanol using an amylase-producing yeast is of interest as an alternative to the conventional fermentation processes, which utilise commercial amylases. Starch is an abundant ... -
Determination of the malting and milling performance of sorghum cultivars
(University of the Free State, 2001-05)English: Consumer demands for sorghum food acceptability stress the need for quality evaluation of milled and malted products. The aim of this study was to determine the milling and malting quality of sorghum cultivars ... -
Fruit quality of South African cactus pear cultivars
(University of the Free State, 2010-06)English: The cultivation of cactus pears requires low input and it has been grown widely in drier areas of South-Africa as fodder crop, particularly for times of serious drought. Cactus pears also serve as a source of ... -
Exploring horizontal gene transfer and phage infections in a South African deep subsurface bacterial population
(University of the Free State, 2017-06)Viruses (more specifically bacteriophages which infect both bacteria and archaea) are the most abundant microorganisms in the ecosystem. In extreme environments such as the deep subsurface, bacteriophages play an important ... -
The effect of irradiation and elevated temperature on the ripening of cheddar cheese
(University of the Free State, 2001-11)English: Proteolysis and lipolysis are important biochemical events in the ripening of cheese to contribute to the development of flavour and texture. The characteristic proteolysis of each type of cheese is brought about ... -
A comparative study of proteolysis in cheddar cheese and yeast-inoculated cheddar cheese during ripening
(University of the Free State, 2000-05)English: Proteolysis is regarded as the most important event in the ripening of Cheddar cheese to contribute to the development of flavour. The characteristic proteolysis of each type of cheese is brought about by the ... -
The production of citric acid by Yarrowia lipolytica when cultivated on edible and waste fats
(University of the Free State, 2003-11)English: Large amounts of edible fat waste (approx. 100 000 tons p.a.) are generated in South Africa when edible fats, mainly sunflower fat, are used in frying processes. When these fats are overexposed to heat a part of ... -
Application of fungi in biotechnological processes for the pulp and paper industry
(University of the Free State, 2001-11)English: Lignin, hemicellulose and cellulose occur together in wood and agricultural wastes that are used in industries such as the pulp and paper industry. Biodegradation could be applied by these industries to save ... -
Yeast diversity in white mould-ripened cheeses
(University of the Free State, 2002-01)Surface mould-ripened cheeses represent a small proportion of world cheese production. However, these cheeses are becoming increasingly popular with the consumer, as there is an increasing demand for it (Gripon, 1987). ... -
Cryopreservation and chemotaxonomy in Saccharomyces meyen ex reess
(University of the Free State, 1998-11)English: The ability to preserve microorganisms can be considered a major . biological achievement. Of special importance is the understanding of the principles of culture preservation with minimal occurrence of contamination, ... -
Participatory development of an indigenous goat cheese product: monitoring of the chemical, nutritional and microbiological quality from milk to cheese
(University of the Free State, 2001-05)English: The milk of indigenous goats has great potential in providing a nutritional food for poverty stricken communities. The aim of this study was to investigate the microbiological (spoilage and pathogenic), chemical ...
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