Factors contributing to sodium dodecyl sulphate (SDS) sedimentation in irrigation wheat

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dc.contributor.advisorVan Biljon, A.
dc.contributor.advisorLabuschagne, M. T.
dc.contributor.advisorWentzel, B. S.
dc.contributor.authorMashamba, Nthabiseng Takalani
dc.date.accessioned2018-01-23T09:56:15Z
dc.date.available2018-01-23T09:56:15Z
dc.date.issued2017-04
dc.description.abstractThe main objective of this study was to determine factors contributing to sodium dodecyl sulphate-sedimentation volume (SDSVOL) in elite wheat breeding lines grown at three different irrigation locations. This was possible through the use of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), mixograph analysis and size exclusion-high performance liquid chromatography (SE-HPLC) techniques. Genotype and environment effects were highly significant for SDSVOL, measured quality characteristics as well as the protein fractions, whereas genotype by environment (GxE) interaction was either significant or not significant for most of the measured variables and highly significant for almost all the protein fractions except the absolute value. Relationships between SDSVOL and other quality characteristics varied in combined and single location analysis of variance. The combined analysis shows a positive relationship between SDSVOL and flour protein content (FPC), high molecular weightglutenin subunits (HMW-GS) and dough characteristics. Protein fractions also revealed positive relationships with SDSVOL and the measured characteristics such as FPC, envelope peak integral (EPI), midline right integral (MRI) and envelope peak time (EPT). Principal component analysis (PCA) reduced 21 quality characteristics to five principle components, which explained 99.83% of total variance of envelope tail integral (ETI), midline left integral (MLI), midline peak integral (MPI), MRI, midline tail integral (MTI), SDSVOL and EPI. The SDSVOL and MRI showed the strongest discriminatory power compared to the other measured quality characteristics. Five of the elite lines (13, 12, 7, 2 and 1) can be recommended to breeders for further evaluation based on their good quality characteristics and strong relationship with some measured quality characteristics.en_ZA
dc.description.sponsorshipDepartment of Educationen_ZA
dc.description.sponsorshipNational Research Funding (NRF)en_ZA
dc.identifier.urihttp://hdl.handle.net/11660/7685
dc.language.isoenen_ZA
dc.publisherUniversity of the Free Stateen_ZA
dc.rights.holderUniversity of the Free Stateen_ZA
dc.subjectSodium sulfateen_ZA
dc.subjectWheat -- Irrigationen_ZA
dc.subjectSDS-PAGEen_ZA
dc.subjectMixograph parametersen_ZA
dc.subjectBread making qualityen_ZA
dc.subjectSDSVOLen_ZA
dc.subjectPCAen_ZA
dc.subjectSE-HPLCen_ZA
dc.subjectWheat -- Breedingen_ZA
dc.subjectDissertation (M.Sc.Agric. (Plant Sciences: Plant Breeding))--University of the Free State, 2017en_ZA
dc.titleFactors contributing to sodium dodecyl sulphate (SDS) sedimentation in irrigation wheaten_ZA
dc.typeDissertationen_ZA
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