Factors contributing to sodium dodecyl sulphate (SDS) sedimentation in irrigation wheat
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Date
2017-04
Authors
Mashamba, Nthabiseng Takalani
Journal Title
Journal ISSN
Volume Title
Publisher
University of the Free State
Abstract
The main objective of this study was to determine factors contributing to sodium dodecyl
sulphate-sedimentation volume (SDSVOL) in elite wheat breeding lines grown at three
different irrigation locations. This was possible through the use of sodium dodecyl
sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), mixograph analysis and size
exclusion-high performance liquid chromatography (SE-HPLC) techniques. Genotype
and environment effects were highly significant for SDSVOL, measured quality
characteristics as well as the protein fractions, whereas genotype by environment (GxE)
interaction was either significant or not significant for most of the measured variables
and highly significant for almost all the protein fractions except the absolute value.
Relationships between SDSVOL and other quality characteristics varied in combined
and single location analysis of variance. The combined analysis shows a positive
relationship between SDSVOL and flour protein content (FPC), high molecular weightglutenin
subunits (HMW-GS) and dough characteristics. Protein fractions also revealed
positive relationships with SDSVOL and the measured characteristics such as FPC,
envelope peak integral (EPI), midline right integral (MRI) and envelope peak time (EPT).
Principal component analysis (PCA) reduced 21 quality characteristics to five principle
components, which explained 99.83% of total variance of envelope tail integral (ETI),
midline left integral (MLI), midline peak integral (MPI), MRI, midline tail integral (MTI),
SDSVOL and EPI. The SDSVOL and MRI showed the strongest discriminatory power
compared to the other measured quality characteristics. Five of the elite lines (13, 12, 7,
2 and 1) can be recommended to breeders for further evaluation based on their good
quality characteristics and strong relationship with some measured quality
characteristics.
Description
Keywords
Sodium sulfate, Wheat -- Irrigation, SDS-PAGE, Mixograph parameters, Bread making quality, SDSVOL, PCA, SE-HPLC, Wheat -- Breeding, Dissertation (M.Sc.Agric. (Plant Sciences: Plant Breeding))--University of the Free State, 2017