Validation of the Cerebrospinal Fluid (CSF) Module of the Siemens ADVIA® 2120i for Automated Cell Counts of Cerebrospinal Fluid
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Date
2019-12
Authors
Kalambi-Matengu, Esther
Journal Title
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Publisher
University of the Free State
Abstract
Introduction: The majority of haematology analysers routinely utilised for whole blood specimens are now equipped with cerebrospinal fluid (CSF) and/or body fluid modules. These automated cell counters are steadily replacing manual microscopy for CSF cell counts. This switch is attributed to the automated analysers’ higher throughput, improved turnaround times, superior precision and higher accuracy compared to manual microscopy. Methods: 46 routine CSF samples were analysed using the ADVIA2120i CSF Module to acquire a red cell count (RCC), white cell count (WCC) and a four-part WCC differential. Results were compared against the mean of two CSF cell counts performed by manual microscopy using the Improved Neubauer chamber haemocytometer. Quantitative method comparison was performed using EP Evaluator® software version 8.0.0 and Microsoft Excel®. The ADVIA2120i was, furthermore, assessed for precision, linearity and carryover. Results: The ADVIA 2120i meets all acceptable claims in terms of precision, and demonstrated acceptable accuracy for RCC, mononuclear and polymorphonuclear cells, compared with the reference method. Linearity results showed that the ADVIA2120i produced results that were proportional after serial dilutions. All parameters were deemed to have acceptable carryover. Conclusions: The ADVIA2120i offers rapid, precise and accurate RCC and WCC for both normal and abnormal cell counts. Our results show that the ADVIA2120i is a suitable alternative to manual microscopy for CSF cell counts and is deemed fit for purpose.
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Keywords
Dissertation (M.Med Sc. (Haematology and Cell Biology))--University of the Free State, 2019, Cerebrospinal fluid, Manual microscopy, ADVIA2120i, Red cell counts, White cell counts