Validation of the Cerebrospinal Fluid (CSF) Module of the Siemens ADVIA® 2120i for Automated Cell Counts of Cerebrospinal Fluid

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dc.contributor.advisorHaupt, Leriska
dc.contributor.advisorCoovadia, Yacoob
dc.contributor.authorKalambi-Matengu, Esther
dc.date.accessioned2022-03-28T15:07:21Z
dc.date.available2022-03-28T15:07:21Z
dc.date.issued2019-12
dc.description.abstractIntroduction: The majority of haematology analysers routinely utilised for whole blood specimens are now equipped with cerebrospinal fluid (CSF) and/or body fluid modules. These automated cell counters are steadily replacing manual microscopy for CSF cell counts. This switch is attributed to the automated analysers’ higher throughput, improved turnaround times, superior precision and higher accuracy compared to manual microscopy. Methods: 46 routine CSF samples were analysed using the ADVIA2120i CSF Module to acquire a red cell count (RCC), white cell count (WCC) and a four-part WCC differential. Results were compared against the mean of two CSF cell counts performed by manual microscopy using the Improved Neubauer chamber haemocytometer. Quantitative method comparison was performed using EP Evaluator® software version 8.0.0 and Microsoft Excel®. The ADVIA2120i was, furthermore, assessed for precision, linearity and carryover. Results: The ADVIA 2120i meets all acceptable claims in terms of precision, and demonstrated acceptable accuracy for RCC, mononuclear and polymorphonuclear cells, compared with the reference method. Linearity results showed that the ADVIA2120i produced results that were proportional after serial dilutions. All parameters were deemed to have acceptable carryover. Conclusions: The ADVIA2120i offers rapid, precise and accurate RCC and WCC for both normal and abnormal cell counts. Our results show that the ADVIA2120i is a suitable alternative to manual microscopy for CSF cell counts and is deemed fit for purpose.en_ZA
dc.identifier.urihttp://hdl.handle.net/11660/11562
dc.language.isoenen_ZA
dc.publisherUniversity of the Free Stateen_ZA
dc.rights.holderUniversity of the Free Stateen_ZA
dc.subjectDissertation (M.Med Sc. (Haematology and Cell Biology))--University of the Free State, 2019en_ZA
dc.subjectCerebrospinal fluiden_ZA
dc.subjectManual microscopyen_ZA
dc.subjectADVIA2120ien_ZA
dc.subjectRed cell countsen_ZA
dc.subjectWhite cell countsen_ZA
dc.titleValidation of the Cerebrospinal Fluid (CSF) Module of the Siemens ADVIA® 2120i for Automated Cell Counts of Cerebrospinal Fluiden_ZA
dc.typeDissertationen_ZA
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