Enzymes from yeast adjuncts in proteolysis during cheddar cheese ripening

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dc.contributor.advisorOsthoff, G.
dc.contributor.advisorDe Wit, M.
dc.contributor.authorAmos, Lize-Mari
dc.date.accessioned2015-07-27T07:09:37Z
dc.date.available2015-07-27T07:09:37Z
dc.date.issued2007-11
dc.description.abstractA great deal of research in cheese technology is devoted towards the manipulation of glycolysis, lipolysis and proteolysis in order to accelerate ripening times or to improve flavour. There is increasing evidence that certain yeast species contribute to flavour and texture development during ripening of certain cheeses, including Cheddar cheese. The addition of yeast cultures as adjunct cultures may accelerate ripening, or lead to a faster development of flavour and taste. This is brought about by an increase in primary proteolysis of the caseins as well as the break down of amino acids into flavour compounds. The respective enzymes involved seem to be proteases with plasmin or plasmin activator activity, and glutamate dehydrogenase. This research reports on the inclusion of yeasts as adjuncts in the processing of Cheddar cheese. Yeasts that expressed both enzyme activities were added as single adjuncts, while combinations of yeasts with singular enzyme activity were employed. Changes during ripening were monitored by sensory and biochemical analyses. The latter includes proteolysis, lipolysis and enzyme activity.en_ZA
dc.identifier.urihttp://hdl.handle.net/11660/682
dc.language.isoenen_ZA
dc.publisherUniversity of the Free Stateen_ZA
dc.rights.holderUniversity of the Free Stateen_ZA
dc.subjectCheesemakingen_ZA
dc.subjectProteolytic enzymesen_ZA
dc.subjectCheddar cheese -- Microbiologyen_ZA
dc.subjectBacterial starter culturesen_ZA
dc.subjectPlasminen_ZA
dc.subjectGlutamate dehydrogenaseen_ZA
dc.subjectYeasten_ZA
dc.subjectDissertation (M.Sc.Agric. (Microbial, Biochemical and Food Biotechnology))--University of the Free State, 2007en_ZA
dc.titleEnzymes from yeast adjuncts in proteolysis during cheddar cheese ripeningen_ZA
dc.typeDissertationen_ZA
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