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dc.contributor.advisorSinclair, Werner
dc.contributor.authorBotha, Anton R.
dc.date.accessioned2017-10-16T08:57:15Z
dc.date.available2017-10-16T08:57:15Z
dc.date.issued2016-02
dc.identifier.urihttp://hdl.handle.net/11660/7251
dc.description.abstractBackground: Polymerase chain reaction (PCR) has been used for many years to detect Mycobacterium tuberculosis (M.tb) DNA in biopsy tissue of erythema induratum (EI)/nodular vasculitis. Studies to ascertain the association between a positive PCR and clinical response to anti-tuberculosis (TB) therapy are lacking. Objectives: Our aim was to determine the association between a favourable clinical response to anti-TB treatment and a positive PCR for M.tb in histologically proven EI. Methods: Twenty-four cases of histologically proven EI were identified that had been biopsied in our department between 1 January 2009 and 31 December 2014. The response to anti-TB therapy was then determined retrospectively, establishing in which patients the subcutaneous nodules of EI had resolved on treatment. Thereafter the formalin-fixed paraffin-embedded tissue sections were sent for PCR. Results: All patients included in our study received anti-TB treatment. The clinical response was favourable in eighteen patients (75%), no response to treatment was observed in five (20,8%) and in one patient (4,1%), who was lost to follow-up, the response could not be determined. The PCR for M.tb was positive in only one sample (4,1%) whereas the other twenty-three samples (95,8%) had a negative PCR for M.tb. Conclusion: The PCR technique on formalin-fixed tissue remains subject to multiple technical pitfalls. Thus only positive results are meaningful, whilst negative results are inconclusive.en_ZA
dc.language.isoenen_ZA
dc.publisherUniversity of the Free Stateen_ZA
dc.subjectPolymerase chain reactionen_ZA
dc.subjectMycobacterium tuberculosis -- Treatmenten_ZA
dc.subjectHistologyen_ZA
dc.subjectTuberculosis -- Treatmenten_ZA
dc.subjectDissertation (M.Med. (Dermatology))--University of the Free State, 2016en_ZA
dc.titleAssociation between a favourable clinical response to anti-tuberculosis treatment and a positive PCR test for mycobacterium tuberculosis in histologically proven erythema induratum/nodular vasculitisen_ZA
dc.typeDissertationen_ZA
dc.rights.holderUniversity of the Free Stateen_ZA


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