Haematology and Cell Biology
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Characterisation of the fibrinolytic system and the Von Willebrand factor-Adamts13 axis in the Chacma baboon
(University of the Free State, 2021-11)Background: The Chacma baboon (Papio ursinus) model of acquired thrombotic thrombocytopenic purpura (aTTP) is ideally suited to investigate novel treatments with potential application in this lethal thrombotic disorder, ... -
Validation of the Cerebrospinal Fluid (CSF) Module of the Siemens ADVIA® 2120i for Automated Cell Counts of Cerebrospinal Fluid
(University of the Free State, 2019-12)Introduction: The majority of haematology analysers routinely utilised for whole blood specimens are now equipped with cerebrospinal fluid (CSF) and/or body fluid modules. These automated cell counters are steadily replacing ... -
Evaluation of the eosin-5-maleimide flow cytometric test and other screening tests in the diagnosis of hereditary spherocytosis
(University of the Free State, 2020-06)Background: Hereditary spherocytosis (HS) is a genetically determined haemolytic anaemia characterised by the spherical shape of affected red blood cells. With limited confirmatory tests currently available in South Africa, ... -
Haemostatic and thrombotic disorders: a journey from bench to bedside
(University of the Free State, 2019-12)This compilation focuses on thrombotic and haemostatic disorders, illustrating my journey from basic research on thrombotic and haemostatic disorders to the differential diagnoses of these disorders. For purposes of clarity ... -
Comparison of ADAMTS13 and Von Willebrand factor antigen levels and activities and plasminogen levels, in the currently available plasma products for the treatment of thrombotic thrombocytopenic purpura in South Africa
(University of the Free State, 2018)Thrombotic thrombocytopenic purpura results from a deficiency in the Von Willebrand factor cleaving protease, ADAMTS13. Treatment involves plasma exchange therapy with either fresh frozen plasma, cryosupernatant, or ... -
Telomeres and telomerase in cancer of esophagus
(University of the Free State, 2001-11)English: Squamous cell carcinoma of the esophagus is a cancer with a high incidence in South Africa. We have investigated the prognostic value of telomerase activity in tumors as well as in nearby normal tissue. Biopsies ... -
Recombinant production and evaluation of a multifunctional haemostatic fusion protein
(University of the Free State, 1999-10)Platelets and coagulation both play a pivotal role in thrombosis, one of the major life-threatening diseases in our society. We have thus experienced a drastic increase in the development of potent and secure ... -
Presence of glyphosate in food products in South Africa of which maize or soybean is the primary constituent
(University of the Free State, 2017-02)English: South Africa is considered a major GM crop producing country. The predominant GM trait in maize and soybean in South Africa is herbicide tolerance. Herbicide tolerant (HT) crops allow for the application of herbicide ... -
Molecular screening of Coloured South African breast cancer patients for the presence of BRCA mutations using high resolution melting analysis
(University of the Free State, 2016-08)English: The populations of South Africa (SA) exhibit a rainbow of genetic diversity due to the high contribution of ancestral genetic admixture. The economic structure of this third world country has limited the exploration ... -
Investigating the genetic profile of the E-cadherin gene in squamous carcinoma of the esophagus
(University of the Free State, 2003-06) -
Co-expression and functional assay of human Rb and E2F1 proteins in yeast
(University of the Free State, 1999-09)The mammalian cell cycle is composed of a myriad interactions occurring in a defined sequence dictated by the flow of entropy. The decision to study the cell cycle requires entry into a world where events take place, not ... -
A yeast-based assay for detection of mutations in the human p16 gene
(University of the Free State, 1999-05)Cyclin-dependent kinases (CDKs) are crucial regulators of the cell cyele. CDKs themselves are subject to control by both cyelins and CDK inhibitors. Among the inhibitors, p 16 is very prominent, since it has been found ... -
Selection and characterization of a novel factor XI inhibiting peptide by using phage display technology
(University of the Free State, 2002-11)English: The role of factor XI in hemostasis can be seen as a combination of a procoagulant action (the formation of fibrin) and an antifibrinolytic action (the protection of fibrin). High levels of factor XI lead to a ... -
Phage display selection of peptide inhibitors of FVIIa and their functional characterisation
(University of the Free State, 2002-06)English: The importance of FVlla and the FVlla/TF complex for the initiation of not only hemostasis but also thrombosis is now generally accepted. It was shown that the blockade of coagulation at the level of FVlla ... -
DNA characterization of the FGA locus in the human genome
(University of the Free State, 2002-11)English: The human alpha fibrinogen (FGA) short tandem repeat locus is found in the long arm of chromosome 4. It is located in the third intron of the alpha fibrinogen gene. This complex highly polymorphic tetranucleotide ... -
Screening for calreticulin mutations in a cohort of patients suspected of having a myeloproliferative neoplasm
(Health and Medical Publishing Group (HMPG), 2016)Background. The discovery of calreticulin (CALR) has shown it to be the second most frequent mutation after the Janus Kinase 2 (JAK2) mutation in myeloproliferative neoplasms (MPNs). Its structure indicates various ... -
The evaluation of tirofiban hydrochloride in a high shear rate arterial thrombosis model in baboons
(University of the Free State, 2009-11)English: Background: Acute coronary syndrome (ACS) is a major cause of mortality and morbidity world-wide, and is responsible for roughly 2.5 million hospital admissions world-wide annually. ACS is commonly associated ... -
Molecular screening for the presence of large deletions or duplications in BRCA using Multiplex ligation-dependent probe amplification in South Africa
(University of the Free State, 2016-07)English: Germline BRCA gene mutations are associated with hereditary breast and ovarian cancers (OVC). Identification of these mutations greatly improves the preventive strategies and management of patients affected with ... -
Molecular screening of the South African Indian population for BRCA1 and BRCA2 using high resolution melting analysis
(University of the Free State, 2016-01)English: The lifetime risk for developing breast cancer within the Indian population of South Africa is one in 17. Disease causing mutations in BRCA1/2 increase the risk of developing this disease by up to 80%. The main ...
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