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dc.contributor.advisorKock, J. L. F.
dc.contributor.advisorSmit, M. S.
dc.contributor.advisorVan Wyk, P. W. J.
dc.contributor.authorSebolai, Olihile Moses
dc.date.accessioned2015-11-09T09:55:32Z
dc.date.available2015-11-09T09:55:32Z
dc.date.issued2004-11
dc.identifier.urihttp://hdl.handle.net/11660/1528
dc.description.abstractEnglish: In this study, the construction of a forecasting model, using intracellular fatty acid composition as indicator, was attempted to assist in the search for yeasts capable of producing 3-hydroxy oxylipins. In order to achieve this, it was first attempted to establish a database mapping the distribution of fatty acids (FAs) associated with the neutral-, glyco- and phospholipid fractions of the 10 species representing the genus Saccharomycopsis. It was possible to identify nine of the 10 species i.e. Saccharomycopsis capsularis, S. crataegensis, S. fibuligera, S. javanensis, S. malanga, S. schoenii, S. selenospora, S. synnaedendra and S vini with the exception of S. fermentans. Saccharomycopsis crataegensis was unique since it produced by far the highest percentage neutral lipids (52.4% w/w) while S. schoenii produced the highest percentage phospholipids (35.9% w/w). All strains produced palmitic- (16:0), stearic- (18:0), oleic- (18:1) and linoleic acid (18:2) in all lipid fractions analysed. The major FAs produced were 18:1 and 18:2, while palmitoleic- (16:1) and a-linolenic acid [18:3 (w-3)] varied between species. Saccharomycopsis capsularis produced the highest percentage 18:2 in the neutral lipid fraction while S. crataegensis, S. malanga and S. selenospora produced the highest percentages of 18:1, 18:0 and, 18:3 (w-3) respectively, in the neutral lipids. Saccharomycopsis vini produced the lowest percentage 16:0 in this fraction. Saccharomycopsis fibuligera and S. schoenii produced the highest percentages of 16:0 and 18:2 respectively in the glycolipid fraction. Saccharomycopsis javanensis and S. synnaedendra produced the highest percentages of 18:1 and 16:1 respectively in the phospholipid fraction. Although it was possible to differentiate between most species using this phenotypic character, these FAs could not be used to predict what kind of 3-OH oxylipins these species are capable of producing. Saccharomycopsis fermentans (novel unidentified 3-OH oxylipin), S. malanga (3-OH 16:0), S. synnaedendra (3-OH 16:0, 3-OH 17:0, 3-OH 18:0, 3-OH 18:1, 3-OH 19:0, 3-OH 19:1, 3-OH 20:0, 3-OH 22:0) and S. vini (3-OH 9:1, 3-OH 10:1) could be separated using this character. Although, S. capsularis and S. javanensis both produced 3-OH 9:1, fatty acids with uneven carbon atoms which may serve as precursors could not be detected in the neutral-, glyco- or phospho-lipid fractions.en_ZA
dc.description.abstractAfrikaans: Hierdie studie het gepoog om ‘n voorspellingsmodel met intrasellulêre vetsuursamestelling as indikator, op te stel. Die doel hiervan was om behulpsaam te wees in die soeke na giste wat 3-hidroksievetsure kan produseer. Ten einde in die doel te slaag is eerstens gepoog om ‘n databasis van die verspreiding van vetsure geassosieer met die neutrale, gliko- en fosfolipiede van 10 spesies van die genus Saccharomycopsis op te stel. Dit was moontlik om nege van die 10 spesies te onderskei, nl. Saccharomycopsis capsularis, S. crataegensis, S. fibuligera, S. javanensis, S. malanga, S. schoenii, S. selenospora, S. synnaedendra en S vini, met die uitsondering van S. fermentans. Saccharomycopsis crataegensis was uniek aangesien dit verreweg die hoogste persentasie neutrale lipiede geproduseer het (52.4% w/w), terwyl S. schoenii die hoogste persentasie fosfolipiede geproduseer het (35.9% w/w). Alle stamme het palmitien- (16:0), stearien- (18:0), oleïn- (18:1) en linolieensuur (18:2) in alle geanaliseerde lipiedfraksies geproduseer. Die hoof vetsure was 18:1 en 18:2, terwyl palmitoleïn- (16:1) en -linoleen [18:3 (-3)] tussen spesies gevarieer het. Saccharomycopsis capsularis het die hoogste persentasie 18:2 in die neutral lipiedfraksie geproduseer, terwyl S. crataegensis, S. malanga en S. selenospora onderskeidelik die hoogste persentasies 18:1, 18:0 en 18:3 (-3) in die neutrale lipiede geproduseer het. Saccharomycopsis vini het die laagste persentasie 16:0 in hierdie fraksie geproduseer. Saccharomycopsis fibuligera en S. schoenii het onderskeidelik die hoogste persentasies 16:0 en 18:2 in die glikolipiedfraksie geproduseer. Saccharomycopsis javanensis en S. synnaedendra het onderskeidelik die hoogste persentasies 18:1 en 16:1 in die fosfolipiedfraksie geproduseer. Alhoewel dit moontlik was om mb.v. hierdie fenotipiese eienskap tussen die meeste spesies te onderskei, kon hierdie vetsure nie gebruik word om te voorspel watter soorte 3-OH vetsure hierdie spesies kan produseer nie. Saccharomycopsis fermentans (nuwe ongeïdentifiseerde 3-OH vetsuur), S. malanga (3-OH 16:0), S. synnaedendra (3-OH 16:0, 3-OH 17:0, 3-OH 18:0, 3-OH 18:1, 3-OH 19:0, 3-OH 19:1, 3-OH 20:0, 3-OH 22:0) en S. vini (3-OH 9:1, 3-OH 10:1) kon onderskei word m.b.v hierdie eienskap. Alhoewel, S. capsularis en S. javanensis albei 3- OH 9:1 geproduseer het, kon vetsure met ‘n onewe getal koolstofatome wat kan dien as voorlopers egter nie in die neutrale, gliko- of fosfolipiede opgespoor word nie.af
dc.description.sponsorshipNational Research Foundation (NRF)en_ZA
dc.language.isoenen_ZA
dc.publisherUniversity of the Free Stateen_ZA
dc.subjectGas chromatography - mass spectrometryen_ZA
dc.subject3-Hydroxy oxylipinsen_ZA
dc.subjectImmunofluorescenceen_ZA
dc.subjectLipidsen_ZA
dc.subjectSaccharomycopsisen_ZA
dc.subjectTaxonomyen_ZA
dc.subjectYeasten_ZA
dc.subjectBioprospectingen_ZA
dc.subjectFatty acidsen_ZA
dc.subjectYeast fungi -- Classificationen_ZA
dc.subjectDissertation (M.Sc. (Microbial, Biochemical and Food Biotechnology))--University of the Free State, 2004en_ZA
dc.titleThe lipid composition of the yeast genus Saccharomycopsis Schiönningen_ZA
dc.typeDissertationen_ZA
dc.rights.holderUniversity of the Free Stateen_ZA


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