Susceptibility tests and predictions of transporter profile in Serratia species
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Date
2022
Authors
Staats, Gunther J.
McCarlie, Samantha J.
Boucher-Van Jaarsveld, Charlotte E.
Bragg, Robert R.
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Disinfectants and biosecurity are critically important to control microbial diseases. Resistance
to disinfectants compromises sectors such as agriculture and healthcare systems. Currently,
efflux pumps are the most common mechanism of antimicrobial resistance. This study aimed to
identify the efflux transporters responsible for disinfectant resistance in a multidrug-resistant isolate
Serratia sp. HRI compared to a susceptible Serratia sp. type strain. An efflux system profile was generated
using the Transporter Automatic Annotation Pipeline (TransAAP) for both isolates. Thereafter,
the efflux pump inhibitors, reserpine (RSP) and carbonyl cyanide 3-chlorophenylhydrazone
(CCCP) were used to reveal the role of efflux pumps in susceptibility to three disinfectants (Didecyldimethylammonium
chloride, HyperCide®, and benzalkonium chloride). Interestingly, the resistant
isolate had fewer efflux systems in total compared to the type strain and fewer efflux systems classified
as resistance efflux pumps. After the addition of RSP, a significant reduction in resistance
capabilities against all three antimicrobials was observed for both isolates. However, CCCP supplementation
produced mixed results with some outcomes suggesting the involvement of the Eagle
effect. This study provides evidence that efflux pumps are responsible for the disinfectant resistance
phenotype of the Serratia species due to the increased susceptibility when efflux pump inhibitors
are added.
Description
Keywords
Disinfectant resistance, Efflux pump, Reserpine, Carbonyl cyanide 3-chlorophenylhydra-zone, Serratia marcescens, Multidrug-resistance
Citation
Staats, G. J., McCarlie, S. J., Boucher-van Jaarsveld, C. E., & Bragg, R. R. (2022). Susceptibility tests and predictions of transporter profile in Serratia species, Microorganisms, 10.2257. https://doi.org/10.3390/microorganisms10112257