Susceptibility tests and predictions of transporter profile in Serratia species

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dc.contributor.authorStaats, Gunther J.
dc.contributor.authorMcCarlie, Samantha J.
dc.contributor.authorBoucher-Van Jaarsveld, Charlotte E.
dc.contributor.authorBragg, Robert R.
dc.date.accessioned2023-03-03T11:52:28Z
dc.date.available2023-03-03T11:52:28Z
dc.date.issued2022
dc.description.abstractDisinfectants and biosecurity are critically important to control microbial diseases. Resistance to disinfectants compromises sectors such as agriculture and healthcare systems. Currently, efflux pumps are the most common mechanism of antimicrobial resistance. This study aimed to identify the efflux transporters responsible for disinfectant resistance in a multidrug-resistant isolate Serratia sp. HRI compared to a susceptible Serratia sp. type strain. An efflux system profile was generated using the Transporter Automatic Annotation Pipeline (TransAAP) for both isolates. Thereafter, the efflux pump inhibitors, reserpine (RSP) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP) were used to reveal the role of efflux pumps in susceptibility to three disinfectants (Didecyldimethylammonium chloride, HyperCide®, and benzalkonium chloride). Interestingly, the resistant isolate had fewer efflux systems in total compared to the type strain and fewer efflux systems classified as resistance efflux pumps. After the addition of RSP, a significant reduction in resistance capabilities against all three antimicrobials was observed for both isolates. However, CCCP supplementation produced mixed results with some outcomes suggesting the involvement of the Eagle effect. This study provides evidence that efflux pumps are responsible for the disinfectant resistance phenotype of the Serratia species due to the increased susceptibility when efflux pump inhibitors are added.en_ZA
dc.description.versionPublisher's versionen_ZA
dc.identifier.citationStaats, G. J., McCarlie, S. J., Boucher-van Jaarsveld, C. E., & Bragg, R. R. (2022). Susceptibility tests and predictions of transporter profile in Serratia species, Microorganisms, 10.2257. https://doi.org/10.3390/microorganisms10112257en_ZA
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/11660/12038
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthor(s)en_ZA
dc.rights.licensehttps://creativecommons. org/licenses/by/4.0/en_ZA
dc.subjectDisinfectant resistanceen_ZA
dc.subjectEfflux pumpen_ZA
dc.subjectReserpineen_ZA
dc.subjectCarbonyl cyanide 3-chlorophenylhydra-zoneen_ZA
dc.subjectSerratia marcescensen_ZA
dc.subjectMultidrug-resistanceen_ZA
dc.titleSusceptibility tests and predictions of transporter profile in Serratia speciesen_ZA
dc.typeArticleen_ZA
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