Regulation of proline accumulation and protein secretion in sorghum under combined osmotic and heat stress

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dc.contributor.authorNgwenya, Samkelisiwe P.
dc.contributor.authorMoloi, Sellwane J.
dc.contributor.authorShargie, Nemera G.
dc.contributor.authorBrown, Adrian P.
dc.contributor.authorChivasa, Stephen
dc.contributor.authorNgara, Rudo
dc.date.accessioned2024-08-21T09:06:32Z
dc.date.available2024-08-21T09:06:32Z
dc.date.issued2024
dc.description.abstractPlants reprogramme their proteome to alter cellular metabolism for effective stress adaptation. Intracellular proteomic responses have been extensively studied, and the extracellular matrix stands as a key hub where peptide signals are generated/processed to trigger critical adaptive signal transduction cascades inaugurated at the cell surface. Therefore, it is important to study the plant extracellular proteome to understand its role in plant development and stress response. This study examined changes in the soluble extracellular sub-proteome of sorghum cell cultures exposed to a combination of sorbitol-induced osmotic stress and heat at 40 °C. The combined stress significantly reduced metabolic activity and altered protein secretion. While cells treated with osmotic stress alone had elevated proline content, the osmoprotectant in the combined treatment remained unchanged, confirming that sorghum cells exposed to combined stress utilise adaptive processes distinct from those invoked by the single stresses applied separately. Reactive oxygen species (ROS)-metabolising proteins and proteases dominated differentially expressed proteins identified in cells subjected to combined stress. ROS-generating peroxidases were suppressed, while ROS-degrading proteins were upregulated for protection from oxidative damage. Overall, our study provides protein candidates that could be used to develop crops better suited for an increasingly hot and dry climate.
dc.description.versionPublisher's version
dc.identifier.citationNgwenya, S. P., Moloi, S. J., Shargie, N. G., Brown, A. P., Chivasa, S., & Ngara, R. (2024). Regulation of Proline Accumulation and Protein Secretion in Sorghum under Combined Osmotic and Heat Stress. Plants, 13(13), 1874. https://doi.org/10.3390/plants13131874
dc.identifier.issn2223-7747 (online)
dc.identifier.urihttps://doi.org/10.3390/plants13131874
dc.identifier.urihttp://hdl.handle.net/11660/12753
dc.language.isoen
dc.publisherMDPI
dc.rights.holderAuthor(s)
dc.rights.licensehttps://www.mdpi.com/about/openaccess
dc.subject𝘚𝘰𝘳𝘨𝘩𝘶𝘮 𝘣𝘪𝘤𝘰𝘭𝘰𝘳
dc.subjectcell suspension cultures
dc.subjectsecreted proteins
dc.subjectsecretome
dc.subjectextracellular matrix
dc.subjectcombined stress
dc.subjectproteomics
dc.subjectiTRAQ
dc.titleRegulation of proline accumulation and protein secretion in sorghum under combined osmotic and heat stress
dc.typeArticle
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