The repurposing of acetylsalicylic acid as a photosensitiser to inactivate the growth of cryptococcal cells

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dc.contributor.authorOgundeji, Adepemi O.
dc.contributor.authorMjokane, Nozethu
dc.contributor.authorFolorunso, Olufemi S.
dc.contributor.authorPohl, Carolina H.
dc.contributor.authorNyaga, Martin M.
dc.contributor.authorSebolai, Olihile M.
dc.date.accessioned2022-01-24T08:22:59Z
dc.date.available2022-01-24T08:22:59Z
dc.date.issued2021
dc.description.abstractPhotodynamic treatment (PDT) is often successful when used against aerobic microbes, given their natural susceptibility to oxidative damage. To this end, the current study aimed to explore the photodynamic action of acetylsalicylic acid (ASA; aspirin, which is commonly used to treat non-infectious ailments), when administered to respiring cryptococcal cells. The treatment of cryptococcal cells, i.e., exposure to 0.5 or 1 mM of ASA in the presence of ultraviolet light (UVL) for 10 min, resulted in a significant (p < 0.05) reduction in the growth of tested cells when compared to non-treated (non-Rx) cells, i.e., no ASA and no UVL. The treated cells were also characterised by diseased mitochondria, which is crucial for the survival of respiring cells, as observed by a significant (p < 0.05) loss of mitochondrial membrane potential (DYM) and significant (p < 0.05) accumulation of reactive oxygen species (ROS) when compared to non-Rx cells. Moreover, the photolytic products of acetylsalicylic acid altered the ultrastructural appearance of treated cells as well as limited the expression levels of the capsular-associated gene, CAP64, when compared to non-Rx cells. The results of the study highlight the potential use of ASA as a photosensitiser that is effective for controlling the growth of cryptococcal cells. Potentially, this treatment can also be used as an adjuvant, to complement and support the usage of current anti-microbial agents.en_ZA
dc.description.versionPublisher's versionen_ZA
dc.identifierhttps://doi.org/10.3390/ph14050404
dc.identifier.citationOgundeji, A.O., Mjokane, N., Folorunso, O.S., Pohl, C.H., Nyaga, M.M., & Sebolai, O.M. (2021). The repurposing of acetylsalicylic acid as a photosensitiser to inactivate the growth of cryptococcal cells. Pharmaceuticals, 14, 404. https://doi.org/10.3390/ph14050404en_ZA
dc.identifier.issn1424-8247 (online)
dc.identifier.urihttp://hdl.handle.net/11660/11366
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthor(s)en_ZA
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/)
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/)
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
dc.subjectAcetylsalicylic acid (ASA, aspirin)en_ZA
dc.subjectCapsuleen_ZA
dc.subjectCAP64en_ZA
dc.subjectCryptococcusen_ZA
dc.subjectMembrane potentialen_ZA
dc.subjectPhotodynamic treatmenten_ZA
dc.subjectPhotosensitiseren_ZA
dc.subjectUltrastructureen_ZA
dc.titleThe repurposing of acetylsalicylic acid as a photosensitiser to inactivate the growth of cryptococcal cellsen_ZA
dc.typeArticleen_ZA
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