Cryptococcal protease(s) and the activation of SARS-CoV-2 spike (S) protein

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dc.contributor.authorMjokane, Nozethu
dc.contributor.authorMaliehe, Maphori
dc.contributor.authorFolorunso, Olufemi S.
dc.contributor.authorOgundeji, Adepemi O.
dc.contributor.authorGcilitshana, Onele M. N.
dc.contributor.authorAlbertyn, Jacobus
dc.contributor.authorPohl, Carolina H.
dc.contributor.authorSebolai, Olihile M.
dc.date.accessioned2022-02-03T09:44:43Z
dc.date.available2022-02-03T09:44:43Z
dc.date.issued2022
dc.description.abstractIn this contribution, we report on the possibility that cryptococcal protease(s) could activate the SARS-CoV-2 spike (S) protein. The S protein is documented to have a unique four-amino-acid sequence (underlined, SPRRAR|S) at the interface between the S1 and S2 sites, that serves as a cleavage site for the human protease, furin. We compared the biochemical efficiency of cryptococcal protease(s) and furin to mediate the proteolytic cleavage of the S1/S2 site in a fluorogenic peptide. We show that cryptococcal protease(s) processes this site in a manner comparable to the efficiency of furin (p > 0.581). We conclude the paper by discussing the impact of these findings in the context of a SARS-CoV-2 disease manifesting while there is an underlying cryptococcal infection.en_ZA
dc.description.versionPublisher's versionen_ZA
dc.identifierhttps://doi.org/10.3390/cells11030437
dc.identifier.citationMjokane, N., Maliehe, M., Folorunso, O.S., Ogundeji, A.O., Gcilitshana, O.M.N., Albertyn, J., Pohl, C.H., & Sebolai, O.M. (2022). Cryptococcal protease(s) and the activation of SARS-CoV-2 spike (S) protein. Cells, 11, 437. https://doi.org/10.3390/cells11030437en_ZA
dc.identifier.issn2073-4409
dc.identifier.urihttp://hdl.handle.net/11660/11394
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthor(s)en_ZA
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/
dc.subjectCryptococcal infectionen_ZA
dc.subjectCryptococcusen_ZA
dc.subjectCryptococcus neoformansen_ZA
dc.subjectFluorogenic peptideen_ZA
dc.subjectFurinen_ZA
dc.subjectProteaseen_ZA
dc.subjectProteolytic cleavageen_ZA
dc.subjectS1/S2 cleavage siteen_ZA
dc.subjectSARS-CoV-2en_ZA
dc.subjectSpike (S) proteinen_ZA
dc.titleCryptococcal protease(s) and the activation of SARS-CoV-2 spike (S) proteinen_ZA
dc.typeArticleen_ZA
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