Impact of three different processing techniques on the strength and structure of juvenile pulmonary homografts

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dc.contributor.authorVan den Heever, Johannes J.
dc.contributor.authorJordaan, C. J.
dc.contributor.authorLewies, Angelique
dc.contributor.authorGoedhals, Jacqueline
dc.contributor.authorBester, Dreyer
dc.contributor.authorBotes, Lezelle
dc.contributor.authorDohmen, Pascal M.
dc.contributor.authorSmit, Francis E.
dc.date.accessioned2022-08-22T12:13:17Z
dc.date.available2022-08-22T12:13:17Z
dc.date.issued2022
dc.description.abstractHomografts are routinely stored by cryopreservation; however, donor cells and remnants contribute to immunogenicity. Although decellularization strategies can address immunogenicity, additional fixation might be required to maintain strength. This study investigated the effect of cryopreservation, decellularization, and decellularization with additional glutaraldhyde fixation on the strength and structure of ovine pulmonary homografts harvested 48 h post-mortem. Cells and cellular remnants were present for the cryopreserved group, while the decellularized groups were acellular. The decellularized group had large interfibrillar spaces in the extracellular matrix with uniform collagen distribution, while the additional fixation led to the collagen network becoming dense and compacted. The collagen of the cryopreserved group was collapsed and appeared disrupted and fractured. There were no significant differences in strength and elasticity between the groups. Compared to cryopreservation, decellularization without fixation can be considered an alternative processing technique to maintain a well-organized collagen matrix and tissue strength of homografts.en_ZA
dc.description.versionPublisher's versionen_ZA
dc.identifierhttps://doi.org/10.3390/polym14153036
dc.identifier.citationVan den Heever, J.J., Jordaan, C., Lewies, A., Goedhals, J., Bester, D., Botes, L., Dohmen, P.M., & Smit, F.E. (2022). Impact of three different processing techniques on the strength and structure of juvenile pulmonary homografts. Polymers, 14, 3036. https://doi.org/10.3390/polym14153036en_ZA
dc.identifier.issn2073-4360
dc.identifier.urihttp://hdl.handle.net/11660/11863
dc.language.isoenen_ZA
dc.publisherMDPIen_ZA
dc.rights.holderAuthor(s)en_ZA
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/
dc.subjectHomograftsen_ZA
dc.subjectIschaemic harvestingen_ZA
dc.subjectDecellularisationen_ZA
dc.subjectCryopreservationen_ZA
dc.subjectGlutaraldehyde-fixationen_ZA
dc.titleImpact of three different processing techniques on the strength and structure of juvenile pulmonary homograftsen_ZA
dc.typeArticleen_ZA
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