High strain diversity among isoniazid-resistant M tuberculosis isolates from the Free State and Northern Cape provinces

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dc.contributor.authorMokhethi, Zac
dc.contributor.authorVan der Spoel-Van Dijk, Anneke
dc.contributor.authorVan der Zanden, Adri
dc.contributor.authorRigouts, Leen
dc.date.accessioned2019-06-14T11:26:56Z
dc.date.available2019-06-14T11:26:56Z
dc.date.issued2005
dc.description.abstractEnglish: This study set out to determine the diversity of the isoniazid-resistant M tuberculosis isolates causing pulmonary tuberculosis in the Free State and Northern Cape provinces of South Africa. Thirty-seven isoniazid-resistant isolates from a nation-wide survey could be fingerprinted by IS6110-based RFLP. Spoligotyping and MIRU-VNTR typing were performed to confirm inconclusive results. The number of IS6110 copies per isolate varied from 2 to 18, with nine (24.3%) isolates harbouring five insertions or fewer. Nine (24.3%) of 37 cases grouped into four RFLP-clusters. The clustered isolates with fewer than five IS6110 copies were all shown to be different when subjected to spoligotyping and MIRU-VNTR typing, whereas the clustering of the high-copy isolates was confirmed. The high degree of diversity among H-resistant isolates in the Free State suggests a low recent transmission rate, but this should be investigated by long-term analyses. For the Northern Cape province, representative sampling is needed before proper conclusions can be drawn.en_ZA
dc.description.abstractDutch: Het objectief is het bepaling van de diversiteit bij isoniazide-resistente M tuberculosis iso- laten die pulmonaire tuberculose veroorzaken in de Vrijstaat en Noordkaap provincies in Zuid Afrika. Zevenendertig isoniazide-resistente isolaten van een nationale survey konden worden getypeerd met IS6110-RFLP. Spoligotypering en MIRU-VNTR typering werden gebruikt als bijkomende subtypering. Het aantal IS6110 copiën per isolaat varieerde tussen 2 en 18, en negen (24.3%) isolaten bevatten vijf of minder inserties. Negen (24.3%) van de 37 gevallen groepeerden in vier RFLP-clusters. De isolaten uit de clusters met minder dan vijf IS6110 inserties werden gedifferentieerd met spoligo-typing en MIRU-VNTR typering, terwijl de hoge-copie clusters werden bevestigd. De hoge mate van diversiteit bij de H-resistente isolaten van de Vrijstaat zou een lage recent transmissie kunnen suggereren, maar moet bevestigd worden met lange-termijn analyses. Voor de Noordkaap provincie, is een representative staalname nodig vooraleer conclusies kunnen getrokken worden.en_ZA
dc.description.versionPublisher's versionen_ZA
dc.identifier.citationMokhethi, Z., Van der Spoel-van Dijk, A., Van der Zanden, A., & Rigouts, L. (2005). High strain diversity among isoniazid-resistant M tuberculosis isolates from the Free State and Northern Cape provinces. Acta Academica, (Supplement 1), 110-127.en_ZA
dc.identifier.issn0587 - 2405 (print)
dc.identifier.issn2415 - 0479 (online)
dc.identifier.urihttp://hdl.handle.net/11660/9790
dc.language.isoenen_ZA
dc.publisherUniversity of the Free Stateen_ZA
dc.rights.holderUniversity of the Free Stateen_ZA
dc.subjectTuberculosisen_ZA
dc.subjectSouth Africaen_ZA
dc.subjectFree Stateen_ZA
dc.subjectNorthern Capeen_ZA
dc.subjectIsoniazid-resistant isolatesen_ZA
dc.titleHigh strain diversity among isoniazid-resistant M tuberculosis isolates from the Free State and Northern Cape provincesen_ZA
dc.typeArticleen_ZA
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AA 2005 Supplementum 1