• Validation of the Cerebrospinal Fluid (CSF) Module of the Siemens ADVIA® 2120i for Automated Cell Counts of Cerebrospinal Fluid 

  Kalambi-Matengu, Esther (University of the Free State, 2019-12)

  Introduction: The majority of haematology analysers routinely utilised for whole blood specimens are now equipped with cerebrospinal fluid (CSF) and/or body fluid modules. These automated cell counters are steadily replacing ...

• Evaluation of the eosin-5-maleimide flow cytometric test and other screening tests in the diagnosis of hereditary spherocytosis 

  Mothi, Hemasha (University of the Free State, 2020-06)

  Background: Hereditary spherocytosis (HS) is a genetically determined haemolytic anaemia characterised by the spherical shape of affected red blood cells. With limited confirmatory tests currently available in South Africa, ...

• Comparison of ADAMTS13 and Von Willebrand factor antigen levels and activities and plasminogen levels, in the currently available plasma products for the treatment of thrombotic thrombocytopenic purpura in South Africa 

  Van Marle, Anneke (University of the Free State, 2018)

  Thrombotic thrombocytopenic purpura results from a deficiency in the Von Willebrand factor cleaving protease, ADAMTS13. Treatment involves plasma exchange therapy with either fresh frozen plasma, cryosupernatant, or ...

• Presence of glyphosate in food products in South Africa of which maize or soybean is the primary constituent 

  Koortzen, B. J. (University of the Free State, 2017-02)

  English: South Africa is considered a major GM crop producing country. The predominant GM trait in maize and soybean in South Africa is herbicide tolerance. Herbicide tolerant (HT) crops allow for the application of herbicide ...

• Molecular screening of Coloured South African breast cancer patients for the presence of BRCA mutations using high resolution melting analysis 

  Oosthuizen, Jaco (University of the Free State, 2016-08)

  English: The populations of South Africa (SA) exhibit a rainbow of genetic diversity due to the high contribution of ancestral genetic admixture. The economic structure of this third world country has limited the exploration ...

• Investigating the genetic profile of the E-cadherin gene in squamous carcinoma of the esophagus 

  Mabina, Boitumelo Desiree (University of the Free State, 2003-06)

• Co-expression and functional assay of human Rb and E2F1 proteins in yeast 

  Wollenschlaeger, Alex (University of the Free State, 1999-09)

  The mammalian cell cycle is composed of a myriad interactions occurring in a defined sequence dictated by the flow of entropy. The decision to study the cell cycle requires entry into a world where events take place, not ...

• A yeast-based assay for detection of mutations in the human p16 gene 

  Botha, Chantal (University of the Free State, 1999-05)

  Cyclin-dependent kinases (CDKs) are crucial regulators of the cell cyele. CDKs themselves are subject to control by both cyelins and CDK inhibitors. Among the inhibitors, p 16 is very prominent, since it has been found ...

• Selection and characterization of a novel factor XI inhibiting peptide by using phage display technology 

  Motloi, Nthabiseng Cecilia (University of the Free State, 2002-11)

  English: The role of factor XI in hemostasis can be seen as a combination of a procoagulant action (the formation of fibrin) and an antifibrinolytic action (the protection of fibrin). High levels of factor XI lead to a ...

• DNA characterization of the FGA locus in the human genome 

  Asfaw, Estifanos Kebede (University of the Free State, 2002-11)

  English: The human alpha fibrinogen (FGA) short tandem repeat locus is found in the long arm of chromosome 4. It is located in the third intron of the alpha fibrinogen gene. This complex highly polymorphic tetranucleotide ...

• The evaluation of tirofiban hydrochloride in a high shear rate arterial thrombosis model in baboons 

  Janse van Rensburg, Walter James (University of the Free State, 2009-11)

  English: Background: Acute coronary syndrome (ACS) is a major cause of mortality and morbidity world-wide, and is responsible for roughly 2.5 million hospital admissions world-wide annually. ACS is commonly associated ...

• Molecular screening for the presence of large deletions or duplications in BRCA using Multiplex ligation-dependent probe amplification in South Africa 

  Moeti, Pakiso James (University of the Free State, 2016-07)

  English: Germline BRCA gene mutations are associated with hereditary breast and ovarian cancers (OVC). Identification of these mutations greatly improves the preventive strategies and management of patients affected with ...

• Molecular screening of the South African Indian population for BRCA1 and BRCA2 using high resolution melting analysis 

  Combrink, H. M. V. E. (University of the Free State, 2016-01)

  English: The lifetime risk for developing breast cancer within the Indian population of South Africa is one in 17. Disease causing mutations in BRCA1/2 increase the risk of developing this disease by up to 80%. The main ...

• Screening black South African females with Type 2 Diabetes Mellitus for mutations in the peroxisome proliferator-activated receptor gamma gene 

  Nienaber, Elzette (University of the Free State, 2016-01)

  English: Type 2 Diabetes Mellitus (T2DM) is a chronic metabolic disorder which is caused by a combination of an inadequate response to insulin secretion and a resistance to insulin action. The peroxisome proliferator-activated ...

• Screening for genetic variants implicated in monogenic forms of hypertension in a hypertensive urban black Free State cohort 

  Smith, Tanja (University of the Free State, 2016-02)

  English: Non-communicable diseases (NCDs), also known as chronic diseases of lifestyle, are the leading cause of death worldwide. Amongst the risk factors for NCDs, hypertension (blood pressure (BP) ≥140/90 mmHg) is one ...

• The application of high-resolution melting curve analysis for the detection of mutations in the MCR-ABL kinase domain of patients with chronic myeloid leukaemia 

  Wienand, Kirsty (University of the Free State, 2011-12)

  CML is a haematological malignancy that is characterized by the BCR-ABL fusion oncogene that encodes a constitutively active tyrosine kinase. The treatment of choice for CML is a tyrosine kinase inhibitor and molecular ...

• Development and application of a real-time PCR method to detect selected single nucleotide polymorphisms associated with hypertension in a black South African population 

  Du Toit, Egardt (University of the Free State, 2014-08)

  English: Hypertension is one of the leading causes of death and disability in the world. In 95% of individuals with hypertension, the condition arises from the interaction of multiple environmental factors with physiological ...

• Characterization of a human inhibitory antibody fragment against tissue factor 

  Vermeulen, Jan-G. (University of the Free State, 2011-11)

  English: Tissue factor is a transmembrane glycoprotein that functions as the primary initiator of coagulation in response to mechanical or chemical damage. Due to its key position within the coagulation cascade it also ...

• The application of real-time quantitative PCR in the diagnostics of chronic myeloid leukaemia 

  Van Deventer, Jacob Jacobus (University of the Free State, 2009-05)

  English: CML is a cancer of the white blood cells and it effects on average one individual in every 100,000. Since it was first described in 1845 by John Hughes Bennett and the subsequent discovery of the Philadelphia ...

• Sequensing of exon 28 of Von Willebrand factor in five patients with type 2 Von Willebrand disease 

  Mothabeng, Maliengoane Sylvia (University of the Free State, 2009-05)

  English: Von Willebrand disease (VWD) is a common bleeding disorder caused by either quantitative (type1 and 3) or qualitative (type 2) defects of von Willebrand factor (VWF). The diagnosis of VWD usually requires a panel ...

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