AA 2005 Supplementum 1

Permanent URI for this collection

http://hdl.handle.net/11660/9663

Browse

Browsing AA 2005 Supplementum 1 by Author "Mokhethi, Zac"

Now showing 1 - 2 of 2
  • Thumbnail Image
    ItemOpen Access
    DNA fingerprinting analyses of M tuberculosis-complex isolates from the Free State, South Africa, as part of a multidisciplinary study
    (University of the Free State, 2005) Van der Spoel-van Dijk, Anneke; Mokhethi, Zac; Khumalo, Macala; Shamputa, Choala; Matebesi, Zacheus; Van Rensburg, Dingie; Portaels, Françoise; Rigouts, Leen
    English: The objective of this study was to serve as a complement to socio-economic analyses of TB patients in a DOTS system, providing microbiological data and documenting the TB population dynamics. Sputum samples were collected from smear-positive TB patients in the Goldfields, Thaba Nchu and Qwaqwa areas. Laboratory analyses comprised the culturing of Mycobacterium tuberculosis isolates and DNA fingerprinting. The primary aim was hampered by problems encountered during specimen sampling, inadequate resources, and a low culture-positivity rate. Nevertheless, the fingerprinting data of a random sample showed a heterogenous TB population, suggesting that reactivation might be an important factor in the area studied. Clustering was the highest in the mining area. Preliminary data from serial isolates also detected possible re-infection during treatment or initial mixed infections in five of the eleven patients.
  • Thumbnail Image
    ItemOpen Access
    High strain diversity among isoniazid-resistant M tuberculosis isolates from the Free State and Northern Cape provinces
    (University of the Free State, 2005) Mokhethi, Zac; Van der Spoel-Van Dijk, Anneke; Van der Zanden, Adri; Rigouts, Leen
    English: This study set out to determine the diversity of the isoniazid-resistant M tuberculosis isolates causing pulmonary tuberculosis in the Free State and Northern Cape provinces of South Africa. Thirty-seven isoniazid-resistant isolates from a nation-wide survey could be fingerprinted by IS6110-based RFLP. Spoligotyping and MIRU-VNTR typing were performed to confirm inconclusive results. The number of IS6110 copies per isolate varied from 2 to 18, with nine (24.3%) isolates harbouring five insertions or fewer. Nine (24.3%) of 37 cases grouped into four RFLP-clusters. The clustered isolates with fewer than five IS6110 copies were all shown to be different when subjected to spoligotyping and MIRU-VNTR typing, whereas the clustering of the high-copy isolates was confirmed. The high degree of diversity among H-resistant isolates in the Free State suggests a low recent transmission rate, but this should be investigated by long-term analyses. For the Northern Cape province, representative sampling is needed before proper conclusions can be drawn.