Browsing Masters Degrees (Microbial, Biochemical and Food Biotechnology) by Title
Now showing items 1-20 of 132
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ADH2 regulation in the yeast Saccharomyces cerevisiae
(University of the Free State, 2004-05)English: The aim of this study was to investigate the ability of ethanol to repress the expression of ADH2 in the genome of Saccharomyces cerevisiae. To achieve this, an expression cassette (ADH2::LacZ) using LacZ as a ... -
Alkane and fatty acid hydroxylating cytochrome P450 monooxygenases in yeast
(University of the Free State, 2008-11)Yarrowia lypolytica, Candida tropicalis, Candida maltosa and Candida cloacae are extensively studied n-alkane degrading yeasts and are widely used in various industrial processes (Madzek et al., 2004, Mobley, 1999). ... -
The Amylostereum symbiont of Sirex noctilio in South Africa
(University of the Free State, 1998-12)English: In Chapter 1 of this thesis, the literature pertaining to the symbiosis between Sirex noctilio and Amy/ostereum areo/atum in the Southern Hemisphere, is reviewed. It is evident from this review that S. noctilio ... -
Antibody fragments as a possible therapeutic treatment for infectious bronchitis in poultry
(University of the Free State, 2018-09)Infectious bronchitis virus (IBV), a coronavirus, is the etiological agent for infectious bronchitis (IB), an acute respiratory disease of poultry. Infectious bronchitis is a notifiable disease, and taking in consideration ... -
Antigenic investigation of genetically different strains of Beak and feather disease virus
(University of the Free State, 2009-01)English: Psittacine beak and feather disease (PBFD) is a dermatological condition that affects both captive and wild psittacine birds worldwide. In Southern Africa, 10 – 20% of breeding stocks are lost due to the disease ... -
Application of amylases for the improvement of water drainage from recycled pulp fibre
(University of the Free State, 2003-05)English: The use of recycled fibre to produce paper is increasing due to improved processes and consumer pressure. However, recycled fibre has slow drainage rates because of fines and residual starch. Slow drainage reduces ... -
Application of fungi in biotechnological processes for the pulp and paper industry
(University of the Free State, 2001-11)English: Lignin, hemicellulose and cellulose occur together in wood and agricultural wastes that are used in industries such as the pulp and paper industry. Biodegradation could be applied by these industries to save ... -
Ascospore release and oxylipin production in the yeast Dipodascopsis
(University of the Free State, 2007-11)The genus Dipodascopsis was extensively studied with regards to reproductive cycles as well as the presence, distribution and function of 3-OH oxylipins. Most of this research was carried out on D. uninucleata var. uninucleata ... -
Biogeochemical and kinetics characterization of sulfate reducing microbial communities enriched from mine drainages
(University of the Free State, 2015-02)English: Acid mine drainage (AMD) or acid rock drainage (ARD) is a global challenge contaminating a lot of the fresh surface and groundwater. The drainage is characterized by low pH, high metal and sulfate concentrations. ... -
A bioinformatic tool for analysing the structures of protein complexes by means of mass spectrometry of cross-linked proteins
(University of the Free State, 2013)English: Multi-subunit protein complexes are involved in many essential biochemical processes including signal transduction, protein synthesis, RNA synthesis, DNA replication and protein degradation. An accurate description ... -
Biological reduction of soluble uranium by an indigenous bacterial community
(University of the Free State, 2013-07)English: Uranium (U) and chromium (Cr) in groundwater are a serious public health concern due to their chemical toxicity. Even so, microorganisms have developed mechanisms which permit them to thrive under previously ... -
Biotransformation of alkanes, alkylbenzenes and their derivatives by genetically engineered Yarrowia lipolytica strains
(University of the Free State, 2005-05)A variety of microorganisms, including yeasts, are capable of utilizing n- alkanes as carbon source (Schmitz et al., 2000; Watkinson & Morgan, 1990). The over expression of P450 genes such as the CYP52 family coding for ... -
Biotransformation of alkylbenzenes and alkylcylcophexanes by genetically engineered Yarrowia lipolytica strains
(University of the Free State, 2008)Y. lipolytica has the ability to utilise hydrophobic hydrocarbons as carbon sources. It is also an attractive host for heterologous expression of cytochrome P450 (CYP) genes. Y. lipolytica strains with CYP genes cloned ... -
Changes in microbial ecology during poultry production
(University of the Free State, 2000-03)Microorganisms, especially pathogens, play an important role in the deterioration of poultry meat and its products causing spoilage or food poisoning. The meat renders an ideal medium for the growth and progression of ... -
Characterization and expression of protein(s) involved in gold nanoparticle formations by Thermus scotoductus SA-01
(University of the Free State, 2010-05)English: Developments in the biosynthesis of nanoparticles have increased significantly during the last few years as a result of the growing interest in the unique properties displayed by nanoparticles. These particles are ... -
Characterization of the Baeyer-Villiger monooxygenase MoxY and closely-related homologues from Aspergillus flavus
(University of the Free State, 2014-02)Aflatoxins are carcinogenic mycotoxins produced by certain species of the Aspergilli, with the most prominent producers being Aspergillus flavus and A. parasiticus. Exposure to aflatoxins causes liver cancer, immune ... -
Characterization of the putative haemagglutinin in haemophilus paragallinarum
(University of the Free State, 2001-12)Haemophilus paragallinarum , the causative agent of infectious coryza (IC), an acute respiratory disease in chickens and fowl, was first isolated in 1931 by De Blieck (1932). The first serious, documented outbreak in South ... -
Charactt[sic]erization of a plasmid conferring NAD independence in Haemophilus paragallinarum
(University of the Free State, 2003-05)English: Members of the family Pasteurellaceae are classified in part by whether or not they require NAD+ supplement for growth on laboratory media. It is known that this phenotype is determined by a plasmid whose presence ... -
Chemical modifications of core histones during exit of stationary phase in Saccharomyces cerevisae
(University of the Free State, 2011-02)The involvement of histone acetylation in facilitating gene expression is well-established, particularly in the case of histones H3 and H4. It was previously shown in Saccharomyces cerevisiae that gene expression was ... -
Classification and spoilage characteristics of Chryseobacterium isolates from fish
(University of the Free State, 2019)The genus Chryseobacterium was initially reported to have only six species and the number has increased rapidly over the years. The genus is represented by a total of 113 species at present. Species belonging to the genus ...