Browsing Doctoral Degrees (Microbial, Biochemical and Food Biotechnology) by Title
Now showing items 1-20 of 56
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Biocatalytic resolution of epoxides: epoxide hydrolases as chiral catalysts for the synthesis of enantiomerically pure epoxides and vic diols from α-olefins
(University of the Free State, 1999-06)English: The synthesis of chiral pharmaceuticals in an enantiopure form had become increasingly important in the last few years. This same trend is now found in the synthesis of agrochemicals. Epoxides, due to their high ... -
Biological synthesis of gold nanoparticles by Thermus scotoductus SA-01
(University of the Free State, 2010-01)English: The usual strategy to prepare gold nanoparticles involves the reduction of a gold salt in solution by various reducing agents in the presence of a stabilizer. These particles are mostly spherical with poor ... -
Biology of botryosphaeria dothidea and sphaeropsis sapinea as endophytes of eucalypts and pines in South Africa
(University of the Free State, 2001-12)English: Botryosphaeria dothidea and Sphaeropsis sapinea both very important pathogens in the South African forestry context. These fungi are well established in the country and contribute substantially to annual losses ... -
Bioremediation of a bleach plant effluent from the pulp and paper industry
(University of the Free State, 2003-11)English: Bleach plant effluent was characterised by physico-chemical methods. The chemistry of the bleach plant effluent was examined to devise effective treatment methods. Effluent contained trace amounts of nitrogen ... -
Characterisation of arsenic hyper-resistance in bacteria isolated from a South African antimony mine
(University of the Free State, 2009-11-20)English: Soil and water sites were sampled at a South African antimony mine with elevated levels of arsenic due to the refining process. Enriched media yielded six pure bacterial cultures able to grow in both arsenite and ... -
The chemical, microbial and sensory evaluation of lucerne (Medicago sativa L.) for human consumption and acceptability
(University of the Free State, 2014-06)English: Lucerne (Medicago sativa L.) has not recently been investigated as an alternative protein source for human consumption in SA. The chemical (degrees Brix, macro- and micro-minerals, protein, AA, dry matter, moisture, ... -
A combined systems biology and genomics approach to the study of metabolism in Kluyveromyces marxianus
(University of the Free State, 2016-10)English: The yeast Kluyveromyces marxianus has become an important micro-organism for industrial applications, as have other non-conventional yeasts. It has the advantages over Saccharomyces cerevisiae (baker’s yeast) ... -
Control of Listeria monocytogenes in an avocado processing facility
(University of the Free State, 2015-11)Listeria monocytogenes contamination of food is a growing concern for the food industry since it is the causative agent of human listeriosis. Despite increased awareness and strict microbiological standards for this ... -
Cytochrome P450 monooxygenases from extremophiles
(University of the Free State, 2012-01)English: Information indicate that CYP450s are prevalent in members of the bacterial phylum Deinococcus-Thermus as well as the archaeal family Halobacteriaceae that belong to the phyulm Euryarchaeota. A property shared by ... -
Determining the canning quality of small seeded white beans (Phaseolus vulgaris L.)
(University of the Free State, 1999-05)English: It is important to the producers, processors and consumers in south Africa to have dry bean cultivars with acceptable canning quality. Therefore, dry bean breeders needs sui table screening methods to evaluate ... -
Development and application of a small-scale canning procedure for the evaluation of small white beans (phaseolus vulgaris)
(University of the Free State, 2004)English: Laboratory canning and evaluation of dry beans are common practices for testing canning quality of cultivars before commercial release to canning industries. Suitable laboratory canning and evaluation procedures ... -
Development of a DNA vaccine for the prevention of Psittacine beak and feather disease
(University of the Free State, 2008-05)Psittacine beak and feather disease (PBFD) is a readily recognisable dermatologic condition in wild and captive psittacines worldwide. It is caused by Beak and feather disease virus (BFDV) which is classified in the family ... -
Development of Yarrowia lipolytica for enhanced production of heterologous proteins
(University of the Free State, 2011-03)English: Yarrowia lipolytica is a non-conventional yeast which is considered to be suitable for production of industrially important proteins at commercial scale. The yeast is non pathogenic and is generally regarded as ... -
Diseases of Acacia mearnsii in South Africa, with particular reference to ceratocystis wilt
(University of the Free State, 1998-12)English: The Acacia mearnsii industry is a relatively small, though very profitable industry in South Africa. Wood derived from A. mearnsii is currently in greater demand than that of either pine or eucalyptus in South ... -
The effect of dietary conjugated linoleic acid supplementation on production efficiency and meat quality of pigs
(University of the Free State, 2014-01)The objectives of this study were to determine the effects of a commercial dietary CLA feed supplement on the production and meat quality parameters of pigs under commercial production conditions. It included the study of ... -
The effect of dietary conjugated linoleic acid supplementation on the physicochemical, nutritional and sensory qualities of pork
(University of the Free State, 2014-05-27)English: Forty eight gilts were fed one of four dietary treatments containing 0, 0.25, 0.5 and 1% CLA, until their weight reached 95 kg and were then slaughtered. There were a lack of significant differences in pig performance ... -
The effect of histone H3 and H4 mutants on the chronological lifespan of Saccharomyces cerevisiae
(University of the Free State, 2015)In this study we maintained histone mutant strains in a batch culture. We observed that some strains significantly decreased at a lower rate in the quiescent population.Some strains disappeared from quiescent population ... -
The effect of sodium reduction on the chemical, microbial and sensory quality of prominent South African processed meat products
(University of the Free State, 2016-08)English: In light of the recent South African regulations limiting the sodium content of processed meat products, the latest draft of these regulations were used to establish to what extent commercial processed meat ... -
Elucidation of African elephant beta casein phosphorylation state and casein micelle structure
(University of the Free State, 2017-01)English: The exact structure of casein micelles still remains a debated subject. While most of the experimental work on cow caseins and casein micelles has provided a wealth of data, data of caseins and casein micelles of ... -
Evaluation of the growth and survival of probiotic microorganisms in commercial bio-yogurt
(University of the Free State, 2000-11)A review of the literature highlighting the importance of the 'therapeutic minimum' and the survival of the probiotic bacteria in fermented milk bioproducts is given in Chapter 2. Special reference is made to the ...