Browsing Doctoral Degrees (Microbial, Biochemical and Food Biotechnology) by Title
Now showing items 34-53 of 56
-
Lipids and ascospore morphology in yeasts
(University of the Free State, 2004-10)Some yeasts produce sexual spores (ascospores) in a variety of shapes and surface ornamentations. These intriguing structures have hitherto been used only in yeast classification. In this study the likely primary function ... -
Lipolytic activity in Geobacillus thermoleovorans GE7: molecular and proteomic charecterization
(University of the Free State, 2010-11)Geobacillus thermoleovorans GE-7 was isolated from the West-Driefontein goldmine in South Africa. It is a Gram-positive rod showing optimal growth at 65°C. This isolate was found to be able to grow on olive oil as the sole ... -
The mechanism of chromate reduction by Thermus scotoductus SA-01
(University of the Free State, 2008-02)English: Recent contamination of the environment with Cr(VI) through various industrial activities is becoming an increasing problem to which microbial Cr(VI) reduction could prove an important alternative solution compared ... -
Microbiological quality and safety of the Zambian fermented cereal beverage: Chibwantu
(University of the Free State, 2014-06)Fermentation has been used for over three thousand years as an effective and inexpensive means to preserve the quality and safety of foods. In Africa most of the traditional foods are fermented at household level before ... -
Mining a South African deep mine metagenome for the discovery of novel biocatalysts
(University of the Free State, 2009-05)English: The construction and screening of gene libraries prepared from DNA directly isolated from environmental samples is a recent and powerful tool for the discovery of new enzymes of biotechnological interest (Gabor ... -
Molecular and physiological aspects of alcohol dehydrogenases in the ethanol metabolism Saccharomyces cerevisiae
(University of the Free State, 2007-05)English: When Saccharomyces cerevisiae is grown on a fermentable carbon source such as glucose, the fermentative alcohol dehydrogenase, ADH I , catalyses the regeneration of NAD+ from NADH and produces ethanol from ... -
Molecular taxonomy and mating type genes in Ceratocystis sensu stricto
(University of the Free State, 1999-01)English: Most species of Ceratocystis sensu stricto are virulent pathogens of a wide variety of plants. In the research presented in this thesis, I have developed a rapid and reliable PCR-based RFLP identification method ... -
Mycotoxin contamination of maize in relation to insect infestation, agricultural practices and agroecology in the Republic of Cameroon
(University of the Free State, 1999-09)English: Maize (Zea mays L.), the staple food crop of the majority of the population of Cameroon, is damaged by insects and diseases from the fields to the stores. As a result, the quantity and the quality of harvested ... -
Oxylipins in Cryptococcus neoformans and related yeasts
(University of the Free State, 2007-11)English: Literature shows that Cryptococcus neoformans is an important human pathogen responsible for many deaths worldwide. To compound this, treatment of cryptococcal infections has over the years been difficult. This ... -
Pathways for anaerobic hydrocarbon degradation in a nitrate reducing consortium
(University of the Free State, 2015-10)English: In summary, the presented data provide comprehensive information to extend our knowledge concerning anaerobic degradation of naphthalene. The results obtained indicated that methylation coupled to fumarate ... -
Phenotypic characterisation of Candida albicans mutants with deletions of arachidonic acid responsive genes
(University of the Free State, 2015-04)English: Candida albicans is a permanent resident of the healthy microbiome of humans. It co-inhabits the host micro-environment with trillions of species which make up the complete microbiota. Host parameters such as ... -
The production of 3-hydroxy fatty acids by the yeast Dipodascopsis uninucleata and its implications
(University of the Free State, 1999-11)English: In 1991, a novel eicosanoid namely 3-hydroxy-5, 8, 11, 14-eicosatetraenoic acid (3-HETE) was uncovered in the yeast Dipodascopsis uninucleata by van Dyk and co-workers. Strikingly, the production of this compound ... -
Prostaglandin E2 production by Candida albicans and Candida dubliniensis
(University of the Free State, 2011-11)English: Arachidonic acid (AA) [20:4(n-6)] is released from infected host cells during Candida albicans infection and may serve as carbon source for yeast growth and as precursor for the production of biologically active ... -
The repurposing of chemical compounds as anti-Cryptococcus drugs
(University of the Free State, 2017-01)English: The manifestation of disseminated cryptococcal infection in HIV-infected individual is a life-threatening infection, with 70% mortality rate in sub-Saharan Africa. People are dying because of the complications ... -
The role of lipids in the flocculation of Saccharomyces cerevisiae
(University of the Free State, 2005-11)English: Although beer production is one of the oldest biotechnologies in the world, a major constraint in brewing remains controlling flocculation. Evidence points towards a possible role of lipids, associated with the ... -
The role of post translational modifications in the regulation of binding of linker histone Hho1 to chromatin in Saccharomyces cerevisiae
(University of the Free State, 2015-02)The eukaryotic genome is functionally organized into a highly ordered nucleoprotein structure, chromatin. Apart from the four nucleosomal core histones, the linker histone is pivotal to fluidity and compaction of chromatin. ... -
The role of post translational modifications in the regulation of binding of linker histone Hho1 to chromatin in Saccharomyces cerevisiae
(University of the Free State, 2015-02)The eukaryotic genome is functionally organized into a highly ordered nucleoprotein structure, chromatin. Apart from the four nucleosomal core histones, the linker histone is pivotal to fluidity and compaction of chromatin. ... -
The survival of yeasts and probiotics as adjunct starters in cheese
(University of the Free State, 2003-11)A literature review dealing with various aspects of cheesemaking and ripening is given in Chapter 1. With respect to starter cultures, the criteria for strain selection as well as the taxonomy of different starter strains ... -
The taxonomy and significance of Chryseobacterium isolates from poultry
(University of the Free State, 2012-01)Species of the genus Chryseobacterium (family Flavobacteriaceae) occur widely in clinical, environmental and industrial ecosystems. In the clinical environment, they are uncommon etiologic agents, but their infections may ... -
Towards unravelling the genome of Avibacterium paragallinarum
(University of the Free State, 2009)Avibacterium paragallinarum is an avian pathogen and has the ability to cause vast economical losses. This bacterium forms part of the Pasteurellaceae family and factors contributing to pathogenicity, immunogenicity and ...