Browsing Masters Degrees (Haematology and Cell Biology) by Issue Date
Now showing items 1-20 of 27
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A yeast-based assay for detection of mutations in the human p16 gene
(University of the Free State, 1999-05)Cyclin-dependent kinases (CDKs) are crucial regulators of the cell cyele. CDKs themselves are subject to control by both cyelins and CDK inhibitors. Among the inhibitors, p 16 is very prominent, since it has been found ... -
Co-expression and functional assay of human Rb and E2F1 proteins in yeast
(University of the Free State, 1999-09)The mammalian cell cycle is composed of a myriad interactions occurring in a defined sequence dictated by the flow of entropy. The decision to study the cell cycle requires entry into a world where events take place, not ... -
DNA characterization of the FGA locus in the human genome
(University of the Free State, 2002-11)English: The human alpha fibrinogen (FGA) short tandem repeat locus is found in the long arm of chromosome 4. It is located in the third intron of the alpha fibrinogen gene. This complex highly polymorphic tetranucleotide ... -
Selection and characterization of a novel factor XI inhibiting peptide by using phage display technology
(University of the Free State, 2002-11)English: The role of factor XI in hemostasis can be seen as a combination of a procoagulant action (the formation of fibrin) and an antifibrinolytic action (the protection of fibrin). High levels of factor XI lead to a ... -
Investigating the genetic profile of the E-cadherin gene in squamous carcinoma of the esophagus
(University of the Free State, 2003-06) -
Construction of cDNA libraries, and the selection and expression of proteins and peptides involved in haemostasis
(University of the Free State, 2004-06)English: The need to find new manners in which to combat cardiovascular disease and associated thrombotic complications, remains a high priority in industrialised countries. Even in third-world countries the implications ... -
"Killer-cell immunoglobulin-like receptor haplotype diversity in three Free State population groups"
(University of the Free State, 2006-11)English: In the foregoing project, an investigation was made into the relative KIR gene frequencies of three South African cohorts. Playing an important part in innate immunity, KIR fill a vital gap between viral onsets ... -
Prevalence of helicobacter pylori and its relation to cytotoxin-associated gene a status in HIV positive and negative haematology patients
(University of the Free State, 2007-10)Abstract not available -
The screening for single nucleotide polymorphisms of CYP3A4 in chronic myelogenous leukemia patients receiving imatinib
(University of the Free State, 2009-05)English: Chronic myelogenous leukaemia (CML) is a malignant clonal disorder that results in the uncontrolled production of white blood cells. This disease is a result of a reciprocal translocation of the long arms of ... -
The application of real-time quantitative PCR in the diagnostics of chronic myeloid leukaemia
(University of the Free State, 2009-05)English: CML is a cancer of the white blood cells and it effects on average one individual in every 100,000. Since it was first described in 1845 by John Hughes Bennett and the subsequent discovery of the Philadelphia ... -
Sequensing of exon 28 of Von Willebrand factor in five patients with type 2 Von Willebrand disease
(University of the Free State, 2009-05)English: Von Willebrand disease (VWD) is a common bleeding disorder caused by either quantitative (type1 and 3) or qualitative (type 2) defects of von Willebrand factor (VWF). The diagnosis of VWD usually requires a panel ... -
The evaluation of tirofiban hydrochloride in a high shear rate arterial thrombosis model in baboons
(University of the Free State, 2009-11)English: Background: Acute coronary syndrome (ACS) is a major cause of mortality and morbidity world-wide, and is responsible for roughly 2.5 million hospital admissions world-wide annually. ACS is commonly associated ... -
Mutational analysis of the Janus Kinase 2 gene in patients with polycythaemia vera, essential thrombocythaemia and primary myelofibrosis
(University of the Free State, 2011-11)All the cells of blood arise from two lineages, the myeloid and the lymphoid lineage. The various cells of blood perform vital functions in the body. These cell counts are closely regulated by regulatory pathways. Mutations ... -
Characterization of a human inhibitory antibody fragment against tissue factor
(University of the Free State, 2011-11)English: Tissue factor is a transmembrane glycoprotein that functions as the primary initiator of coagulation in response to mechanical or chemical damage. Due to its key position within the coagulation cascade it also ... -
The application of high-resolution melting curve analysis for the detection of mutations in the MCR-ABL kinase domain of patients with chronic myeloid leukaemia
(University of the Free State, 2011-12)CML is a haematological malignancy that is characterized by the BCR-ABL fusion oncogene that encodes a constitutively active tyrosine kinase. The treatment of choice for CML is a tyrosine kinase inhibitor and molecular ... -
The effect of inflammatory cytokines and coagulation factors on Von Willebrand factor synthesis and cleavage
(University of the Free State, 2012-02)When injured, endothelial cells secrete inflammatory cytokines such as interleukin-6 (IL-6), interleukin-8 (IL-8), and tumour necrosis factor-α (TNF-α). These inflammatory cytokines stimulate the endothelial release of ... -
Microparticles derived from stimulation of human umbilical endothelium
(University of the Free State, 2013)English: Endothelial microparticle reseach is currently a very novel and exciting topic in the field of haemosistasis and thrombosis. The role of microparticles in inflammatory and thrombotic disorders is however not ... -
Genotypic and expression analysis of CYP3A4 and CYP3A5 in patients with chronic myeloid leukaemia
(University of the Free State, 2013-01)Chronic myeloid leukaemia (CML) is a haematological malignancy characterised by the BCR-ABL fusion oncogene which encodes for a constitutively active tyrosine kinase. Imatinib mesylate is a tyrosine kinase inhibitor that ... -
Development and application of a real-time PCR method to detect selected single nucleotide polymorphisms associated with hypertension in a black South African population
(University of the Free State, 2014-08)English: Hypertension is one of the leading causes of death and disability in the world. In 95% of individuals with hypertension, the condition arises from the interaction of multiple environmental factors with physiological ... -
Thrombogenecity of decellularized and re-endothelialized baboon arteries
(University of the Free State, 2015-02)English: The use of decellularized biological scaffolds for the reconstruction of small-diameter (< 6 mm) vascular grafts has been the focus of tissue engineering studies. These biological materials constructed through ...