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dc.contributor.advisorMeiring, S. M.
dc.contributor.advisorBurt, F. J.
dc.contributor.advisorVan Heerden, E.
dc.contributor.authorVermeulen, Jan-G.
dc.date.accessioned2017-08-17T10:22:24Z
dc.date.available2017-08-17T10:22:24Z
dc.date.issued2017-01
dc.identifier.urihttp://hdl.handle.net/11660/6584
dc.description.abstractEnglish: Recognising the potential in tissue factor inhibition as a novel approach to antithrombotic therapy, our laboratory utilized phage display technology to select a human single chain antibody fragment from the Tomlinson I + J Human Single Fold Phage Libraries which functionally inhibits human tissue factor. Although the initial findings were promising the further characterisation of the tissue factor inhibiting scFv was hampered by low proteins yields as well as financial complication associated with the initial purification methods. In this study, the production of functional antibody was improved through the use of in vitro refolding- and cold shock expression methods. The protein structure and inhibition mechanism was characterised by means of in silico modelling. The improved expression of functional TFI-scFv opens several different pathways for the future characterisation and study of the structure and interaction of TFI-scFv with tissue factor.en_ZA
dc.description.abstractAfrikaans: Ter erkenning in die potensiaal in weefsel faktor inhibisie as 'n nuwe benadering tot anti-trombotiese terapie, het ons laboratorium gebruik gemaak van faagvertooning tegnologie om 'n menslike enkele ketting teenliggaam fragment vanuit die Tomlinson I + J Human single fold library te isoleer wat menslike weefsel faktor inhibeer. Hoewel die aanvanklike bevindings belowend was, was die verdere karakterisering van die antiliggaam bemoeilik deur lae proteïen vlakke asook finansiële komplikasies wat verband hou met die aanvanklike suiwerings metodes. In hierdie studie, is die produksie van funksionele teenliggaam verbeter deur gebruik te maak van in vitro hervouing en koue skok proteïen uitdrukking metodes. Die proteïenstruktuur en inhibisie meganisme is geidentifiseer deur middel van in silico modellering. Die verbeterde uitdrukking van funksionele antiliggaam open verskillende roetes vir die toekomstige karakterisering en studie van die struktuur en interaksie van die antiliggaam met weefsel factor.af
dc.language.isoenen_ZA
dc.publisherUniversity of the Free Stateen_ZA
dc.subjectThrombolytic therapyen_ZA
dc.subjectThromboplastinen_ZA
dc.subjectBlood coagulation factorsen_ZA
dc.subjectBlood -- Coagulationen_ZA
dc.subjectThesis (Ph.D. (Haematology and Cell Biology))--University of the Free State, 2017en_ZA
dc.titlePreparation and in vitro characterisation of an anti-tissue factor single chain variable fragmentsen_ZA
dc.typeThesisen_ZA
dc.rights.holderUniversity of the Free Stateen_ZA


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