Doctoral Degrees (Microbial, Biochemical and Food Biotechnology)
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Recent Submissions
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The production of 3-hydroxy fatty acids by the yeast Dipodascopsis uninucleata and its implications
(University of the Free State, 1999-11)English: In 1991, a novel eicosanoid namely 3-hydroxy-5, 8, 11, 14-eicosatetraenoic acid (3-HETE) was uncovered in the yeast Dipodascopsis uninucleata by van Dyk and co-workers. Strikingly, the production of this compound ... -
Determining the canning quality of small seeded white beans (Phaseolus vulgaris L.)
(University of the Free State, 1999-05)English: It is important to the producers, processors and consumers in south Africa to have dry bean cultivars with acceptable canning quality. Therefore, dry bean breeders needs sui table screening methods to evaluate ... -
A combined systems biology and genomics approach to the study of metabolism in Kluyveromyces marxianus
(University of the Free State, 2016-10)English: The yeast Kluyveromyces marxianus has become an important micro-organism for industrial applications, as have other non-conventional yeasts. It has the advantages over Saccharomyces cerevisiae (baker’s yeast) ... -
Expression of avian pathogenic Escherichia coli (APEC) virulence factors, Iss and HlyF, as potential sub-unit vaccine candidates
(University of the Free State, 2017-06)Avian pathogenic Escherichia coli (APEC) is the causative agent of colibacillosis in poultry and leads to economic losses in the poultry industry. Due to rising concerns of antibiotic resistance and antibiotic carry-over ... -
Molecular and physiological aspects of alcohol dehydrogenases in the ethanol metabolism Saccharomyces cerevisiae
(University of the Free State, 2007-05)English: When Saccharomyces cerevisiae is grown on a fermentable carbon source such as glucose, the fermentative alcohol dehydrogenase, ADH I , catalyses the regeneration of NAD+ from NADH and produces ethanol from ... -
Biology of botryosphaeria dothidea and sphaeropsis sapinea as endophytes of eucalypts and pines in South Africa
(University of the Free State, 2001-12)English: Botryosphaeria dothidea and Sphaeropsis sapinea both very important pathogens in the South African forestry context. These fungi are well established in the country and contribute substantially to annual losses ... -
Evaluation of the growth and survival of probiotic microorganisms in commercial bio-yogurt
(University of the Free State, 2000-11)A review of the literature highlighting the importance of the 'therapeutic minimum' and the survival of the probiotic bacteria in fermented milk bioproducts is given in Chapter 2. Special reference is made to the ... -
Utilisation of edible oils and GLA production by Mucor in the presence of acetate
(University of the Free State, 1998-10)English: Surveys launched across South Africa indicate that many frying establishments abuse their frying oils and fats during the frying process, resulting in degradation and concomitant production of potentially ... -
The repurposing of chemical compounds as anti-Cryptococcus drugs
(University of the Free State, 2017-01)English: The manifestation of disseminated cryptococcal infection in HIV-infected individual is a life-threatening infection, with 70% mortality rate in sub-Saharan Africa. People are dying because of the complications ... -
Elucidation of African elephant beta casein phosphorylation state and casein micelle structure
(University of the Free State, 2017-01)English: The exact structure of casein micelles still remains a debated subject. While most of the experimental work on cow caseins and casein micelles has provided a wealth of data, data of caseins and casein micelles of ... -
Utilization of wood-decay fungi for biokraft pulping of softwood
(University of the Free State, 1999-05)English: The forest products industry is one of the most important earners of foreign exchange for South Africa. The major focus of the industry is the production of pulp with an annual capacity of 2,4 million tons. Wood ... -
Diseases of Acacia mearnsii in South Africa, with particular reference to ceratocystis wilt
(University of the Free State, 1998-12)English: The Acacia mearnsii industry is a relatively small, though very profitable industry in South Africa. Wood derived from A. mearnsii is currently in greater demand than that of either pine or eucalyptus in South ... -
Mycotoxin contamination of maize in relation to insect infestation, agricultural practices and agroecology in the Republic of Cameroon
(University of the Free State, 1999-09)English: Maize (Zea mays L.), the staple food crop of the majority of the population of Cameroon, is damaged by insects and diseases from the fields to the stores. As a result, the quantity and the quality of harvested ... -
Factors associated with coniothyrium canker of Eucalyptus in South Africa
(University of the Free State, 1999-05)English: English: In chapter one of this thesis, the literature pertaining to the genus Coniothyrium and its importance in plant pathology, is reviewed. Special attention is given to Coniothyrium species associated with ... -
Biocatalytic resolution of epoxides: epoxide hydrolases as chiral catalysts for the synthesis of enantiomerically pure epoxides and vic diols from α-olefins
(University of the Free State, 1999-06)English: The synthesis of chiral pharmaceuticals in an enantiopure form had become increasingly important in the last few years. This same trend is now found in the synthesis of agrochemicals. Epoxides, due to their high ... -
Molecular taxonomy and mating type genes in Ceratocystis sensu stricto
(University of the Free State, 1999-01)English: Most species of Ceratocystis sensu stricto are virulent pathogens of a wide variety of plants. In the research presented in this thesis, I have developed a rapid and reliable PCR-based RFLP identification method ... -
Genome sequencing of the extremophile Thermus scotoductus SA-01 and expression of selected genes
(University of the Free State, 2009-05)Thermus scotoductus SA-01 is an extremophile that was isolated from groundwater samples at 3.2 km depth in a South African gold mine and has previously been shown to grow using nitrate, Fe(III), Mn(IV) or Sº as terminal ... -
Development of Yarrowia lipolytica for enhanced production of heterologous proteins
(University of the Free State, 2011-03)English: Yarrowia lipolytica is a non-conventional yeast which is considered to be suitable for production of industrially important proteins at commercial scale. The yeast is non pathogenic and is generally regarded as ... -
The effect of sodium reduction on the chemical, microbial and sensory quality of prominent South African processed meat products
(University of the Free State, 2016-08)English: In light of the recent South African regulations limiting the sodium content of processed meat products, the latest draft of these regulations were used to establish to what extent commercial processed meat ... -
The survival of yeasts and probiotics as adjunct starters in cheese
(University of the Free State, 2003-11)A literature review dealing with various aspects of cheesemaking and ripening is given in Chapter 1. With respect to starter cultures, the criteria for strain selection as well as the taxonomy of different starter strains ...
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