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dc.contributor.authorSchabort, Du Toit W. P.
dc.contributor.authorLetebele, Precious K.
dc.contributor.authorSteyn, Laurinda
dc.contributor.authorKilian, Stephanus G.
dc.contributor.authorDu Preez, James C.
dc.date.accessioned2016-08-25T12:16:34Z
dc.date.available2016-08-25T12:16:34Z
dc.date.issued2016
dc.identifier.citationDu Toit, W. P., Letebele, P. K., Steyn, L., Kilian, S. G., & du Preez, J. C. (2016). Differential RNA-seq, Multi-Network Analysis and Metabolic Regulation Analysis of Kluyveromyces marxianus Reveals a Compartmentalised Response to Xylose. PloS one, 11(6), e0156242. DOI:10.1371/journal.pone.0156242en_ZA
dc.identifier.issn1932-6203 (print)
dc.identifier.urihttp://hdl.handle.net/11660/3955
dc.description.abstractWe investigated the transcriptomic response of a new strain of the yeast Kluyveromyces marxianus, in glucose and xylose media using RNA-seq. The data were explored in a number of innovative ways using a variety of networks types, pathway maps, enrichment statistics, reporter metabolites and a flux simulation model, revealing different aspects of the genome-scale response in an integrative systems biology manner. The importance of the subcellular localisation in the transcriptomic response is emphasised here, revealing new insights. As was previously reported by others using a rich medium, we show that peroxisomal fatty acid catabolism was dramatically up-regulated in a defined xylose mineral medium without fatty acids, along with mechanisms to activate fatty acids and transfer products of β-oxidation to the mitochondria. Notably, we observed a strong up-regulation of the 2-methylcitrate pathway, supporting capacity for odd-chain fatty acid catabolism. Next we asked which pathways would respond to the additional requirement for NADPH for xylose utilisation, and rationalised the unexpected results using simulations with Flux Balance Analysis. On a fundamental level, we investigated the contribution of the hierarchical and metabolic regulation levels to the regulation of metabolic fluxes. Metabolic regulation analysis suggested that genetic level regulation plays a major role in regulating metabolic fluxes in adaptation to xylose, even for the high capacity reactions, which is unexpected. In addition, isozyme switching may play an important role in re-routing of metabolic fluxes in sub- cellular compartments in K. marxianus.en_US
dc.language.isoenen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.titleDifferential RNA-seq, multi-network analysis and metabolic regulation analysis of Kluyveromyces marxianus reveals a compartmentalised response to xyloseen_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's versionen_US
dc.rights.holderAuthors hold copyrighten_ZA


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