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Development of species-specific polymerase chain reaction (PCR), real-time PCR and loop mediated isothermal amplifications (LAMP) assays for detection of Anaplamsa marginale strains in South Africa
(University of the Free State, 2012-12)
Anaplasma marginale is a virulent intra-erythrocytic pathogen that causes bovine anaplasmosis, its closely related species, Anaplasma centrale causes mild sickness. The pathogen is transmitted biologically by tick vectors ...
The species composition and bio-ecology of Culicoides spp. frequenting livestock in the central Free State, South Africa
(University of the Free State, 2012-07)
English: Culicoides midges are involved in the transmission of a variety of pathogens, the most economically important of these are the orbiviruses that cause African horse sickness (AHSV) and bluetongue (BTV). The ...
Development of molecular diagnostic methods (LAMP and PCR) for detection of Haemonchus contortus, Fasciola SPP and Trichostrongylus SPP infections in livestock
(University of the Free State, 2012-12)
Helminths belonging to the genera Haemonchus and Trichostrongylus as well as those of the genus Fasciola are the causative agents of various helminthiases in animals and sometimes in humans. In both host types infections ...
Advances in the systematics and ecology of African Corinnidae spiders (Arachnida: Araneae), with emphasis on the Castianeirinae
(University of the Free State, 2012-01)
English: The Corinnidae is one of 76 families of spiders (Arachnida: Araneae) presently
recognised in the Afrotropical Region. By the end of the last century their taxonomy and
systematics had been very poorly studied ...
Characterisation of treated domestic wastewater and its potential use for small scale urban agriculture in Bulawayo: balancing health and environmental needs
(University of the Free State, 2014-03)
The use of urban wastewater for agriculture crop production is receiving renewed attention in most parts of the world due to the increasing scarcity of water. Water scarcity has placed pressure on the ability of households ...
Revision of the South African geogarypidae (arachnida: Pseudoscorpiones)
(University of the Free State, 2015-01)
English: Despite a recent order-level revision published by Harvey (1992b), where, through the use of 126 morphological characters, 24 families were recognized, detailed morphological and ecological data is still sorely ...
Taxonomic review of the genus Histotylenchus Siddiqi, 1971 (Nematoda: Belonolaimidae) in South Africa
(University of the Free State, 2011-11)
English: A diverse and abundant nematode fauna is known to exist in South Africa with plant parasitic nematodes being the most representative. Seven genera belonging to the family Belonolaimidae Whitehead, 1960 are known ...
Insect-fungal interactions in tree nut crop orchards in South Africa, with specific reference to pecans
(University of the Free State, 2014-01)
English: This study investigates insect-fungal-plant interactions on tree-nut crops (pistachio, walnut and pecan) in South Africa. The main aim was to determine whether insects occurring in tree-nut crop orchards are ...
Assessment and improvement of molecular diagnosis of Theileria parva of African buffalo (Syncerus caffer) in Southern Africa
(University of the Free State, 2015-10-30)
English: Buffalo-adapted Theileria parva causes Corridor disease in cattle. Strict control measures therefore apply to the movement of buffalo in South Africa and include mandatory testing of buffalo for the presence of ...
Comparison of real-time polymerase chain reaction with the conventional PCR assay for the diagnosis of Theileria parva in South Africa
(University of the Free State, 2015-08-04)
Theileria parva (T. parva) is transmitted from carrier buffalo to cattle causing
Corridor disease in cattle. The 989/990 conventional Polymerase Chain
Reaction (PCR) assay used for the detection of T. parva is labour-intensive ...