Microbial, Biochemical and Food Biotechnology
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The production of 3-hydroxy fatty acids by the yeast Dipodascopsis uninucleata and its implications
(University of the Free State, 1999-11)English: In 1991, a novel eicosanoid namely 3-hydroxy-5, 8, 11, 14-eicosatetraenoic acid (3-HETE) was uncovered in the yeast Dipodascopsis uninucleata by van Dyk and co-workers. Strikingly, the production of this compound ... -
Changes in microbial ecology during poultry production
(University of the Free State, 2000-03)Microorganisms, especially pathogens, play an important role in the deterioration of poultry meat and its products causing spoilage or food poisoning. The meat renders an ideal medium for the growth and progression of ... -
Determining the canning quality of small seeded white beans (Phaseolus vulgaris L.)
(University of the Free State, 1999-05)English: It is important to the producers, processors and consumers in south Africa to have dry bean cultivars with acceptable canning quality. Therefore, dry bean breeders needs sui table screening methods to evaluate ... -
The utilisation of used and other fats by fungi
(University of the Free State, 2000-09)English: In 1997, Jeffery and co-workers discovered that when Mucor circinelloides t. circinelloides CBS 108.16 was cultivated on 30g/1 sunflower oil and 10g/1 sodium acetate, an improved utilisation of the oil, doubling ... -
Mucoralean fungi present in soil from arid regions in South Africa
(University of the Free State, 1999-11)English: The aim of the first part of the study was to investigate the ecological niche of mucoralean fungi in arid soil, with specific reference to the position these fungi occupy in the biogeochemical cycle of nitrogen. ... -
Ophiostoma species from hardwood sources in South Africa
(University of the Free State, 2001-10)English: The ophiostomatoid fungi are an economically important group of fungi, known for their ability to stain sapwood and cause tree diseases. In recent years, certain species in the group have also been considered as ... -
The growth kinetic characterisation of Saccharomyces cerevisiae strains transformed with amylase genes
(University of the Free State, 2002-03)English: The direct fermentation of starch to ethanol using an amylase-producing yeast is of interest as an alternative to the conventional fermentation processes, which utilise commercial amylases. Starch is an abundant ... -
Determination of the malting and milling performance of sorghum cultivars
(University of the Free State, 2001-05)English: Consumer demands for sorghum food acceptability stress the need for quality evaluation of milled and malted products. The aim of this study was to determine the milling and malting quality of sorghum cultivars ... -
Fruit quality of South African cactus pear cultivars
(University of the Free State, 2010-06)English: The cultivation of cactus pears requires low input and it has been grown widely in drier areas of South-Africa as fodder crop, particularly for times of serious drought. Cactus pears also serve as a source of ... -
A combined systems biology and genomics approach to the study of metabolism in Kluyveromyces marxianus
(University of the Free State, 2016-10)English: The yeast Kluyveromyces marxianus has become an important micro-organism for industrial applications, as have other non-conventional yeasts. It has the advantages over Saccharomyces cerevisiae (baker’s yeast) ... -
Expression of avian pathogenic Escherichia coli (APEC) virulence factors, Iss and HlyF, as potential sub-unit vaccine candidates
(University of the Free State, 2017-06)Avian pathogenic Escherichia coli (APEC) is the causative agent of colibacillosis in poultry and leads to economic losses in the poultry industry. Due to rising concerns of antibiotic resistance and antibiotic carry-over ... -
Exploring horizontal gene transfer and phage infections in a South African deep subsurface bacterial population
(University of the Free State, 2017-06)Viruses (more specifically bacteriophages which infect both bacteria and archaea) are the most abundant microorganisms in the ecosystem. In extreme environments such as the deep subsurface, bacteriophages play an important ... -
Molecular and physiological aspects of alcohol dehydrogenases in the ethanol metabolism Saccharomyces cerevisiae
(University of the Free State, 2007-05)English: When Saccharomyces cerevisiae is grown on a fermentable carbon source such as glucose, the fermentative alcohol dehydrogenase, ADH I , catalyses the regeneration of NAD+ from NADH and produces ethanol from ... -
Biology of botryosphaeria dothidea and sphaeropsis sapinea as endophytes of eucalypts and pines in South Africa
(University of the Free State, 2001-12)English: Botryosphaeria dothidea and Sphaeropsis sapinea both very important pathogens in the South African forestry context. These fungi are well established in the country and contribute substantially to annual losses ... -
Evaluation of the growth and survival of probiotic microorganisms in commercial bio-yogurt
(University of the Free State, 2000-11)A review of the literature highlighting the importance of the 'therapeutic minimum' and the survival of the probiotic bacteria in fermented milk bioproducts is given in Chapter 2. Special reference is made to the ... -
The effect of irradiation and elevated temperature on the ripening of cheddar cheese
(University of the Free State, 2001-11)English: Proteolysis and lipolysis are important biochemical events in the ripening of cheese to contribute to the development of flavour and texture. The characteristic proteolysis of each type of cheese is brought about ... -
A comparative study of proteolysis in cheddar cheese and yeast-inoculated cheddar cheese during ripening
(University of the Free State, 2000-05)English: Proteolysis is regarded as the most important event in the ripening of Cheddar cheese to contribute to the development of flavour. The characteristic proteolysis of each type of cheese is brought about by the ... -
The production of citric acid by Yarrowia lipolytica when cultivated on edible and waste fats
(University of the Free State, 2003-11)English: Large amounts of edible fat waste (approx. 100 000 tons p.a.) are generated in South Africa when edible fats, mainly sunflower fat, are used in frying processes. When these fats are overexposed to heat a part of ... -
Application of fungi in biotechnological processes for the pulp and paper industry
(University of the Free State, 2001-11)English: Lignin, hemicellulose and cellulose occur together in wood and agricultural wastes that are used in industries such as the pulp and paper industry. Biodegradation could be applied by these industries to save ... -
Yeast diversity in white mould-ripened cheeses
(University of the Free State, 2002-01)Surface mould-ripened cheeses represent a small proportion of world cheese production. However, these cheeses are becoming increasingly popular with the consumer, as there is an increasing demand for it (Gripon, 1987). ...
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