Improved separation of polymeric and monomeric protein fractions using a high resolution column
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Date
2017
Authors
Phakela, Keneuoe
Journal Title
Journal ISSN
Volume Title
Publisher
University of the Free State
Abstract
The main objective of this study was to test the Yarra-SEC 4000 analytical column (300
x 7.80 mm) against the BioSep-SEC s4000 narrow bore column (300 x 4.60 mm) that
was used by most researchers up to now, for separation of wheat gluten proteins, using
SE-HPLC. The relationship between protein fractions and a range of quality
characteristics were then determined. The trials were conducted in the three production
areas of South Africa representing the dryland summer rainfall, dryland winter rainfall
and irrigated regions. These regions each have specific cultivars developed for their
specific conditions. The Yarra-SEC 4000 analytical column yielded nine profile peaks
compared to the five of the BioSep-SEC s4000 narrow bore column. Highly significant
differences were found between the protein fractions for the irrigated region and the
winter rainfall cultivars. Very few significant differences were seen for protein fractions
between cultivars in the dryland summer rainfall region. The GLI%p correlated
positively with dough rheological characteristics in cultivars of the irrigated region. The
UPP positively correlated with dough rheological parameters for cultivars of the irrigated
region. No significant correlations were observed between UPP and dough rheological
characteristics in the dryland winter rainfall cultivars. The irrigated region cultivars
presented more significant correlations between protein fractions and quality
characteristics. Significant correlations were obtained between SDSVOL and protein
fractions for the winter rainfall region cultivars. No significant correlations were obtained
between SDSVOL and protein fractions for the irrigated and dryland summer rainfall
cultivars. In this study it was observed that Yarra-SEC 4000 analytical column can be
employed successfully in the separation of wheat proteins.
Description
Keywords
BioSep-SEC s4000 narrow bore column, Quality characteristics, SEHPLC, Yarra-SEC 4000 analytical column, Gluten, Wheat, Field crops, Grain, Dissertation (M.Sc. (Plant Sciences: Plant Breeding))--University of the Free State, 2017