Now showing items 1-4 of 4
Heterologous expression of cytochrome P450 monooxygenases in different ascomycetous yeasts
(University of the Free State, 2012-07)
Cytochrome P450 monooxygenases (P450) are a diverse, ubiquitous family of important enzymes which catalyze a variety of activities. Their outstanding abilities to hydroxylate non-activated hydrocarbons make them attractive ...
Identification, cloning and heterologous expression of fungal vanillyl-alcohol oxidases
(University of the Free State, 2012-01)
English:There are currently only two confirmed fungal vanillyl-alcohol oxidases (VAOs), one from Penicillium simplicissimum (here called PsVAO) and one from Byssochlamys fulva. Only the gene sequence of PsVAO is available. ...
Evaluation of Yarrowia lipolytica as a host for cytochrome P450 monooxygenase expression
(University of the Free State, 2006-06)
Biohydroxylation reactions are catalyzed by various types of hydroxylating enzymes (Ayala and Torres, 2004) which include dioxygenases, lipooxygenases as well as CYP450 monooxygenases. These particular hydroxylation reactions ...
Mining a South African deep mine metagenome for the discovery of novel biocatalysts
(University of the Free State, 2009-05)
English: The construction and screening of gene libraries prepared from DNA directly isolated from environmental samples is a recent and powerful tool for the discovery of new enzymes of biotechnological interest (Gabor ...